FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052142
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGGGATCCCTGAGCG34910.3317993198636686No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30480.289694737022189No Hit
GTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAAGA30100.2860830572299176No Hit
CCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACG29250.27800429979983693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27810.26431793427122957No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27500.26137156391437655No Hit
CTATATTACTGTGCGAGAAGTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTCTCCGT27120.2577598841221052No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23930.2274407827080375No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21280.2022540683671976No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20550.19531584139783412No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19720.18742717237787296No Hit
CCCCTGTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGGGATCCCT19570.18600150930197637No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACT19560.18590646509691658No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19500.18533619986655792No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19420.18457584622607975No Hit
GGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGACTTAC19010.18067903381862904No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19010.18067903381862904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18740.17811284028201516No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGGAAAGCAAAGTTTC18440.17526151413022195No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18370.17459620469480355No Hit
ATATTACTGTGCGAGAAGTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTCTCCGTCT18010.1711746133126517No Hit
GGGTGGCCATGTTCCCAGAGTTGGAGCCAAGGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTGTCATCAGACA17910.17022417126205397No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17420.1655670052141251No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17420.1655670052141251No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17330.16471160736858714No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17100.16252559065221234No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCAC16760.15929408768018005No Hit
CCCCAGGAGAGACGGCCAGACTTACCTGTGGGGGAGACAACATTGGAGAT16750.1591990434751203No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16440.1562526731182673No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16410.15596754050308798No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGC16320.15511214265755002No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16120.15321125855635456No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15930.15140541866021887No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACAGCCTCATCT15900.15112028604503955No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15500.14731851784264863No Hit
GACTTACCTGTGGGGGAGACAACATTGGAGATGAGGCTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC15400.14636807579205088No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15400.14636807579205088No Hit
TGGCCACCCTGACCGTCAGTGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCACGTGTGGCATACTTCTA15370.14608294317687157No Hit
GCCCTCAGGGATCCCTGAGCGATTCCTTGGCTCCAACTCTGGGAACATGG15370.14608294317687157No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15330.14570276635663248No Hit
GCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGA15300.14541763374145317No Hit
CAGTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAA15280.1452275453313336No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTTATTTTCAGAAACTTTGCT14910.141710909744122No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14640.1391447162075081No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.13800418574679083No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14520.13800418574679083No Hit
CTATTACTGTCACGTGTGGCATACTTCTACTAATCAGGGGGTAATCGGCG14450.13733887631137243No Hit
GTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTC14200.1349627711848781No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA14080.1338222407241608No Hit
ACATTGGAGATGAGGCTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCGTGTCTGATGACA13900.1321114450330849No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13520.1284997652408135No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13520.1284997652408135No Hit
CGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACAGCC13480.12811958842057442No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC13320.12659888113961804No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13300.12640879272949848No Hit
GTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAG13170.12517321806372145No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC13110.1246029528333628No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13100.12450790862830302No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCACTGACGGTCAGGGT13070.1242227760131237No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13040.12393764339794439No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12980.12336737816758575No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12970.12327233396252597No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12760.12127640565627072No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12740.12108631724615118No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12680.12051605201579255No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAATTGGGTTTTC12600.11975569837531436No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCCACTGACGGTCAGGGTGG12340.11728454904376025No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12120.11519357653244525No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12090.11490844391726593No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11890.11300755981607044No Hit
GGCCAGGCCCCTGTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGG11880.11291251561101068No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11680.11101163150981522No Hit
GTGTCATCAGACACGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTA11530.10958596843391863No Hit
GGCCTGGAGTGGCTGGCCCTTATTTCATTTGATGGAAGTATCATCCATTA11460.10892065899850019No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11340.10778012853778292No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGG11270.1071148191023645No Hit
GATTAGTAGAAGTATGCCACACGTGACAGTAATAGTCGGCCTCATCCCCG11260.10701977489730474No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11240.10682968648718519No Hit
CTCCTGTGCAGCCTCTGGATTTATTTTCAGAAACTTTGCTTTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGC11230.10673464228212542No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11220.10663959807706565No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11160.10606933284670701No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11080.10530897920622882No Hit
CAATTATACTTCTCGCACAGTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAAGAGTGTG11040.10492880238598971No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10970.1042634929505713No Hit
GTTAAGAGGTATCGAATGTCAGATGCAATTGAGACAGTCTGGCGGAGGCG10970.1042634929505713No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10800.10264774146455516No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10790.1025526972594954No Hit
CCCTGACCGTCAGTGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT10750.1021725204392563No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10710.1017923436190172No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10630.10103198997853903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTC150.00219545270.2349949
GTGGTAT1500.045.071411
TGGTATC1700.041.747412
GGTATCA5200.041.6043781
ACGGGTA350.0014794438.68781316
GTATCAA14250.038.6665231
GTATACC350.001489853838.6326331
AATGGTA4700.036.6761370
TATCAAC15550.035.4255332
GTATAGG400.002869231633.803561
ATCAACG16350.033.07053
TCAACGC16650.032.4746364
CAACGCA16800.032.3873755
AACGCAG17150.031.7309346
TACGGGT1209.822543E-1131.0175615
GACAGGT1001.7680577E-830.4231991
TATACCT450.005113253430.0404645
ACGCAGA18100.029.8872787
AGAGTAC18350.029.31536511
GACTGTT604.8870523E-429.26458249
Produced by FastQC (version 0.11.5)