FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119822
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGACCGCCAGTGACATTGGTTTTCATGACTATGTCTCCTGGTACCAACACCACCCTGCCAAGGC45220.40381417761037025No Hit
GTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTG37530.3351425494408933No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTC35990.3213903638256794No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34730.3101385755950499No Hit
CTATATGACTTACTACTTTGACCACTGGGGCCTGGGAACCCAGGTCACCGTCTCCTCCGCATCCCCGACCAGCCC34620.30915627662253464No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC32080.28647410034809107No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31880.2846881022162451No Hit
GATTATTACTGCAGCTCATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT31370.2801338069800379No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAAT31010.27691901034271516No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTG29160.2603985276231401No Hit
GTACCAACACCACCCTGCCAAGGCCCCCAAACTCATCATTTATGATGTCAATAATCGGCCCTCTGGGCTATCTAG29040.25932692874403257No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27740.2477179408870338No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27580.24628914238155708No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGGCTAGATAGCCCAGAGGGCCGATT27210.24298504583764205No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26940.24057394835965001No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25700.2295007599422051No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25590.22851846096968984No Hit
CCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC25210.22512506451918252No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24620.21985637003023697No Hit
GTCTGGCACCACGGCCTCGTTGACCATCTTTGGGCTCCAGGCTGAGGACG24580.21949917040386774No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATGGTCAACGAGG23610.21083707946441488No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23480.20967618067871502No Hit
GTGTTGGTACCAGGAGACATAGTCATGAAAACCAATGTCACTGGCGGTCCCAGTGCAGGAGATGGTGACCGACTG23170.2069078835743538No Hit
TCTTTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT22620.2019963887117774No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAA22480.20074619001948527No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22340.1994959913271931No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22180.1980671928217163No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21630.19315569795913995No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21470.19172689945366317No Hit
ACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC21290.1901195011350018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21290.1901195011350018No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21070.18815490318997127No Hit
GGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCAC21020.18770840365700978No Hit
ATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTTGACCA20780.18556520589879463No Hit
GTCATATAGTGACCACTCCAAAAATTAGGGGTCCCCTCTCTTGAACAGTA20600.18395780758013328No Hit
CATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCC20420.18235040926147192No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20390.18208250954169503No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20370.18190390972851042No Hit
GGGCTATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTT20090.17940351234392607No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCT19890.17761751421208014No Hit
AGATAGCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTTGGGGG19480.17395621804179592No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19380.17306321897587296No Hit
CTCCTGGGCAGTCGGTCACCATCTCCTGCACTGGGACCGCCAGTGACATT19240.17181302028358078No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19180.171277220844027No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19090.17047352168469632No Hit
GTGCAGGAGATGGTGACCGACTGCCCAGGAGACCCAGACACGGAGGCAGG18960.16931262289899646No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGC18790.1677945244869274No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18400.16431182812982778No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCAC17460.1559176369101518No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16980.1516312413937215No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16570.1479699452234373No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16460.146987646250922No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTGCACTGGGACCG16430.1467197465311451No Hit
GCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTTGGGGGCCTTGGCAGGGTGGTGTTGGTACCAGGAGA16130.14404074933337618No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15610.13939715419057672No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACT15200.1357358580202925No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15060.13448565932800033No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14920.13323546063570818No Hit
GCCCAAAGATGGTCAACGAGGCCGTGGTGCCAGACTTGGAGCCAGAGAAG14780.131985261943416No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14540.12984206418520086No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14450.12903836502587018No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGC14250.12725236689402422No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13980.12484126941603219No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13920.1243054699764784No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13690.12225157212485556No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG13670.12207297231167095No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13670.12207297231167095No Hit
TCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCT13570.12117997324574799No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13450.12010837436664042No Hit
GTAATAGACGGCTGTGTCCTCGGCTTCCAGACTGTTCATTTGAAGATAGG13430.11992977455345581No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13390.11957257492708662No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC13320.11894747558094053No Hit
ACACCACCCTGCCAAGGCCCCCAAACTCATCATTTATGATGTCAATAATCGGCCCTCTGGGCTATCTAGCCGCTT13210.11796517660842527No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG13140.11734007726227919No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13020.11626847838317161No Hit
GTTTGGGGGCCTTGGCAGGGTGGTGTTGGTACCAGGAGACATAGTCATGA13000.11608987856998702No Hit
CGGCTAGATAGCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTT12880.11501827969087944No Hit
GCTCATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTG12880.11501827969087944No Hit
GTCTATTACTGTTCAAGAGAGGGGACCCCTAATTTTTGGAGTGGTCACTA12790.11421458053154877No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGC12760.11394668081177188No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12530.11189278296014901No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12400.11073188417444915No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12340.11019608473489537No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT12300.10983888510852619No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12290.10974958520193387No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11950.10671338837779576No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTACCTGGGTTTTC11900.10626688884483426No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11850.10582038931187278No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11710.10457019061958062No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11620.10376649146024994No Hit
CTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATGGTCAACGAGGCCGTGGTGC11590.10349859174047303No Hit
CCTCTGGGCTATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTTGACCATCTTTGGGCTCCAGG11590.10349859174047303No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11520.10287349239432694No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11380.1016232937020348No Hit
GCCTGGAGCCCAAAGATGGTCAACGAGGCCGTGGTGCCAGACTTGGAGCC11380.1016232937020348No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11260.10055169482292721No Hit
GCACTGGGACCGCCAGTGACATTGGTTTTCATGACTATGTCTCCTGGTAC11250.10046239491633491No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTGCA11240.10037309500974263No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11210.10010519528996571No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11210.10010519528996571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)