FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119822
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGACCGCCAGTGACATTGGTTTTCATGACTATGTCTCCTGGTACCAACACCACCCTGCCAAGGC44800.40006358153349375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38170.3408577434628003No Hit
GTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTG37890.33835734607821605No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTC36830.3288915559794324No Hit
CTATATGACTTACTACTTTGACCACTGGGGCCTGGGAACCCAGGTCACCGTCTCCTCCGCATCCCCGACCAGCCC32010.285849001001945No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC29870.2667388209911933No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29800.26611372164504715No Hit
GATTATTACTGCAGCTCATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT28630.25566563257374836No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAAT28130.25120063724413344No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27820.2484323401397722No Hit
GTACCAACACCACCCTGCCAAGGCCCCCAAACTCATCATTTATGATGTCAATAATCGGCCCTCTGGGCTATCTAG27620.24664634200792626No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTG26900.24021674873328083No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26470.23637685274981202No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26280.23468015452455837No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25890.23119745816745874No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGGCTAGATAGCCCAGAGGGCCGATT25770.2301258592883512No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24210.21619507385995276No Hit
GGGCTATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTT23850.21298027722263002No Hit
GTCTGGCACCACGGCCTCGTTGACCATCTTTGGGCTCCAGGCTGAGGACG23800.21253377768966852No Hit
GTGTTGGTACCAGGAGACATAGTCATGAAAACCAATGTCACTGGCGGTCCCAGTGCAGGAGATGGTGACCGACTG23710.21173007853033787No Hit
CCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC23690.21155147871715327No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23430.20922968114575352No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23280.20789018254686906No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC23070.2060148845084308No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22740.20306798759088499No Hit
GTCATATAGTGACCACTCCAAAAATTAGGGGTCCCCTCTCTTGAACAGTA22660.2023535883381466No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATGGTCAACGAGG22540.20128198945903902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22530.20119268955244674No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21940.19592399506350114No Hit
GGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCAC21400.19110180010751707No Hit
TCTTTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT21210.18940510188226342No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20830.18601170543175613No Hit
ACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC20790.18565450580538692No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20510.1831541084208026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20040.1789570128109646No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAA19910.17779611402526474No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19590.1749385170143112No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGC19320.17252741953631917No Hit
ATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTTGACCA19250.1719023201901731No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19100.1705628215912886No Hit
CTCCTGGGCAGTCGGTCACCATCTCCTGCACTGGGACCGCCAGTGACATT18710.167080125234189No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCT18630.16636572598145064No Hit
CATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCC18570.16582992654189682No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18250.16297232953094332No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17990.16065053195954357No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17870.159578933080436No Hit
AGATAGCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTTGGGGG17850.1594003332672514No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17760.15859663410792071No Hit
GTGCAGGAGATGGTGACCGACTGCCCAGGAGACCCAGACACGGAGGCAGG17450.1558283370035595No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCAC16920.15109544195416771No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTGCACTGGGACCG16400.14645184681136822No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16290.14546954783885296No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16190.14457654877292997No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16180.14448724886633768No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15710.14029015325649968No Hit
GCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTTGGGGGCCTTGGCAGGGTGGTGTTGGTACCAGGAGA15480.13823625540487686No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15440.13787905577850765No Hit
GTAATAGACGGCTGTGTCCTCGGCTTCCAGACTGTTCATTTGAAGATAGG15070.13457495923459264No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14860.1326996611961544No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG14780.131985261943416No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14220.12698446717424733No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14010.12510916913580908No Hit
GCCCAAAGATGGTCAACGAGGCCGTGGTGCCAGACTTGGAGCCAGAGAAG13960.1246626696028476No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13960.1246626696028476No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13770.12296597137759394No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC13770.12296597137759394No Hit
TCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCT13690.12225157212485556No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACT13640.12180507259189406No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC13370.11939397511390203No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13330.11903677548753283No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13140.11734007726227919No Hit
GCTCATATACAGGCATCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTG12930.11546477922384094No Hit
GTTTGGGGGCCTTGGCAGGGTGGTGTTGGTACCAGGAGACATAGTCATGA12930.11546477922384094No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGC12890.11510757959747174No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12810.11439318034473335No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGC12760.11394668081177188No Hit
ACACCACCCTGCCAAGGCCCCCAAACTCATCATTTATGATGTCAATAATCGGCCCTCTGGGCTATCTAGCCGCTT12510.11171418314696444No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGGTCACCATCTCCTGCA12450.11117838370741065No Hit
GTGTTGATGCCTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC12380.11055328436126456No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12210.1090351859491955No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTGTTGATGCCTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG12150.10849938650964171No Hit
GTCTATTACTGTTCAAGAGAGGGGACCCCTAATTTTTGGAGTGGTCACTA12070.10778498725690334No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTC11930.10653478856461117No Hit
CCTCTGGGCTATCTAGCCGCTTCTCTGGCTCCAAGTCTGGCACCACGGCCTCGTTGACCATCTTTGGGCTCCAGG11860.10590968921846508No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11790.105284589872319No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11670.10421299099321142No Hit
CGGCTAGATAGCCCAGAGGGCCGATTATTGACATCATAAATGATGAGTTT11570.10331999192728844No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11550.10314139211410386No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11550.10314139211410386No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCTTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT11540.10305209220751155No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11530.10296279230091925No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11420.10198049332840398No Hit
CTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAAAGATGGTCAACGAGGCCGTGGTGC11350.10135539398225789No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11300.1009088944492964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)