FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813323
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCAAGTACA31120.3826278120746616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29680.36492266909948445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28110.3456191451612705No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21850.2686509541719587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21520.26459352557348065No Hit
GAATTCACCCTCACCATCAGCAACCTGCAGCCTGATGATTCTGCAACTTA19670.2418473349456489No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19650.24160143018210478No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19210.236191525384134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18440.2267241919876851No Hit
ATGTATTACTGTGCGAGAGAGGTGCCCGGGTTAGCCTCTACCTTTGACTG17360.21344533475630223No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17100.2102485728302286No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCAAGTA16960.2085272394854197No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16720.20557638232289016No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16560.20360914421453713No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16490.20274847754213268No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16480.20262552516036061No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15990.1966008584535295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15810.1943877155816324No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14770.18160066787733778No Hit
GTTGGAGACAGAGTCACCATCATTTGCCGGGCCAGTCAGAATATTGATACCTGGTTGGCCTGGTATCAGCAGAAA14670.18037114405961716No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14490.17815800118772No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14120.17360876306215367No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCATTTGCCG14030.1725021916262051No Hit
CCCTAAGACTCTCCTGTGTAGACTCTGGATTCACCTTTAGTGAATATTGG13800.16967428684544764No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13630.16758409635532256No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCATTTGCCGGGCCAGTCAGA13390.16463323919279302No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13200.1622971439391238No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12870.1582397153406457No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12860.15811676295887364No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12730.15651838199583684No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12570.1545511438874838No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACCCTCACCATCAGCAACCTGCAGCCTGATGATTCTG12540.15418228674216763No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12440.15295276292444698No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11970.14717400098116No Hit
GGACTGGAGTGGGTGGCCAACATAAAGGACGATGGAAGTCAGACAAACTA11820.14532971525457905No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11770.14471495334571874No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11700.1438542866733143No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11570.14225590571027746No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11520.14164114380141715No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCCATGCTTCTGGCAGTAGTAAGTTG11310.1390591437842038No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATATTCAC11300.13893619140243177No Hit
GTATTACTGTGCGAGAGAGGTGCCCGGGTTAGCCTCTACCTTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT10730.13192790564142415No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGTCAAGTGTGGCAT10670.13119019135079177No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10590.13020657229661525No Hit
ACTTACTACTGCCAGAAGCATGGGACTTTTGGCCAGGGGACCAAGCTGGA10230.12578028655282095No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10080.12393600082624No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9650.11864904841004126No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9600.11803428650118096No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9500.11680476268346032No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCATTTGCCGGGCCAGTCAGAATATTGATAC9320.11459161981156318No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCGCTGAGC9290.114222762666247No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9260.1138539055209308No Hit
GAATATTGATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGTGCTCCTGATCTATAAGGCGT9100.11188666741257779No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8920.10967352454068063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8840.10868990548650412No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT8820.10844400072296001No Hit
ACCTTGATGAGACCCCACTTTGTAAATTAGACGCCTTATAGATCAGGAGCACAGGGGCTTTCCCTGGTTTCTG8790.10807514357764382No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8770.10782923881409968No Hit
CTCCTGTGTAGACTCTGGATTCACCTTTAGTGAATATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGA8700.10696857214169524No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGGTGGAGT8690.10684561975992318No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8630.10610790546929079No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8600.1057390483239746No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG8600.1057390483239746No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8590.10561609594220256No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8520.10475542926979811No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8450.10389476259739365No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8450.10389476259739365No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8340.10254228639790097No Hit
GATCTATAAGGCGTCTAATTTACAAAGTGGGGTCTCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC8320.10229638163435682No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8310.10217342925258475No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCGCTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG8230.10118981019840827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)