FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813323
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCAAGTACA30490.3748818120230216No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28170.34635685945190287No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24960.30688914490307045No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23750.2920119067086508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20690.2543884778863994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20570.2529130493051346No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18140.2230356205345232No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17880.21983885860844954No Hit
GAATTCACCCTCACCATCAGCAACCTGCAGCCTGATGATTCTGCAACTTA17480.214920763337567No Hit
ATGTATTACTGTGCGAGAGAGGTGCCCGGGTTAGCCTCTACCTTTGACTG17020.2092649537760521No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16660.20483866803225778No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16580.20385504897808127No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16570.2037320965963092No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGCAAGTA16010.19684676321707364No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14970.18405971551277905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14760.18147771549556574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14650.18012523929607302No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14490.17815800118772No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14420.17729733451531557No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14120.17360876306215367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14060.17287104877152126No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13990.17201038209911682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13560.16672342968291812No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13150.1616823820302635No Hit
GTTGGAGACAGAGTCACCATCATTTGCCGGGCCAGTCAGAATATTGATACCTGGTTGGCCTGGTATCAGCAGAAA13000.15983809630368256No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12900.1586085724859619No Hit
CCCTAAGACTCTCCTGTGTAGACTCTGGATTCACCTTTAGTGAATATTGG12570.1545511438874838No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12330.1516002867249543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11970.14717400098116No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCATTTGCCG11760.14459200096394667No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11670.1434854295279981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11590.14250181047382157No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11410.14028866760192446No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCATTTGCCGGGCCAGTCAGA11350.13955095331129205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11340.13942800092952No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACCCTCACCATCAGCAACCTGCAGCCTGATGATTCTG11290.1388132390206597No Hit
GGACTGGAGTGGGTGGCCAACATAAAGGACGATGGAAGTCAGACAAACTA10990.13512466756749777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10910.1341410485133213No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10820.1330344770773727No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCCATGCTTCTGGCAGTAGTAAGTTG10810.13291152469560064No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10500.12910000086066667No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10410.1279934294247181No Hit
GTATTACTGTGCGAGAGAGGTGCCCGGGTTAGCCTCTACCTTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGT10370.12750161989762984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10110.12430485797155619No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATATTCAC9890.1215999055725708No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9860.12123104842725461No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9720.1195097150824457No Hit
GGGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGAGACCCCA9430.11594409601105589No Hit
ACCTTGATGAGACCCCACTTTGTAAATTAGACGCCTTATAGATCAGGAGCACAGGGGCTTTCCCTGGTTTCTG9400.1155752388657397No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9370.1152063817204235No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGTCAAGTGTGGCAT9340.1148375245751073No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9310.11446866742979112No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.11446866742979112No Hit
ACTTACTACTGCCAGAAGCATGGGACTTTTGGCCAGGGGACCAAGCTGGA9050.11127190550371746No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9050.11127190550371746No Hit
CTCCTGTGTAGACTCTGGATTCACCTTTAGTGAATATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGA8830.10856695310473208No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8770.10782923881409968No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG8530.10487838165157015No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8530.10487838165157015No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCGCTGAGC8490.10438657212448191No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8260.10155866734372446No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8180.10057504828954794No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8160.10032914352600382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)