FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286539
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC85900.6676828296693688No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTACAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAAT58730.45649607201958126No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58510.45478605778759906No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG57950.4504332942880084No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57440.44646917038659534No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTG55070.42804765343297013No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC53500.4158443700501889No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTACAGTTCTGCTGCTTGTATATGAGCTG51440.3998324186052658No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG45840.35630478360935813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG44930.3492315429225232No Hit
CTCCTGGACAGTCGATCTCCATCTCCTGCACTGGAACCAGCAGTGACGTT44890.348920631243981No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC44130.34301330935167923No Hit
GTACAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC42650.3315095772456179No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42290.32871137213873813No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41020.31883992634502334No Hit
GTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG39260.3051598124891667No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39230.30492662873026No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38710.30088477690921145No Hit
GAACTGTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC38700.30080704898957594No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG38140.29645428548998515No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC37350.29031377983877676No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35550.27632275430437786No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT34770.27025997657280504No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34650.2693272415371784No Hit
GCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG33920.26365310340378334No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT33610.2612435378950813No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGAACTGTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT33560.26085489829690356No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33290.2587562444667437No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32930.25595803935986394No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTC32730.254403480967153No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32350.2514498200210021No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29750.23124056091575923No Hit
GAATAGTACAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCT29710.23092964923721707No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29510.22937509084450608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29070.22595506238054192No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28830.22408959230928874No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC28040.21794908665808033No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27630.2147622419530228No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26650.20714490582873896No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26560.206445354552019No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26180.20349169360586813No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25900.2013153118560728No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG25550.19859483466882852No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25220.1960298133208554No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25050.19470843868705107No Hit
GACTCAGCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCT24520.19058885894636696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24500.19043340310709586No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24120.18747974216094498No Hit
GCCCTGACTCAGCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTC23750.18460380913442967No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTACAGTTCTGCTGC22930.1782301197243146No Hit
GTCGATCTCCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTTTGTCTCCTGGTACCAACAACA22870.17776375220650134No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAGCGACTAGAAACCCCTGAGGGCCGA22720.17659783341196808No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22340.1736441724658172No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22330.17356644454618167No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21960.17069051151966633No Hit
GTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC21720.16882504144841315No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21590.167814578493151No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20990.16315090331501805No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20730.16112997740449375No Hit
GCTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAGT20110.1563108463870897No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19780.15374582503911657No Hit
CAGCAGAACTGTACTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19740.15343491336057438No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19490.15149171536968564No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19260.14970397321806803No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC19050.14807168690572148No Hit
AGTCTGCCCTGACTCAGCCTGGCTCCGTGTCTGGGTCTCCTGGACAGTCG18940.1472166797897304No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT18790.1460507609951972No Hit
CCTTGGTCCCTCCGCCGAATAGTACAGTTCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG18790.1460507609951972No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18720.14550666555774835No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18640.14488484220066394No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC18600.14457393052212175No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18540.14410756300430846No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18430.14325255588831742No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18320.14239754877232638No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18280.14208663709378416No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC18090.14060980662070874No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17810.13843342487091337No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17270.1342361172105937No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17130.133147926335696No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17100.13291474257678934No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT16950.13174882378225614No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16490.12817333947902085No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC16310.12677423692558096No Hit
CCCCTGAGGGCCGACTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA16080.12498649477396331No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15990.1242869434972434No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15840.12312102470271014No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCACTGGAACCAG15710.12211056174744801No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATAGTACAGTTCTG15690.12195510590817689No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15530.12071145919400812No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT15060.1170582469711373No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15050.11698051905150175No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14990.11651415153368845No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT14870.11558141649806186No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGGCTCCGTGTCTGGGT14830.11527050481951964No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14690.11418231394462197No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG14600.11348276266790203No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC14510.11278321139118208No Hit
GTCCAGGAGACCCAGACACGGAGCCAGGCTGAGTCAGGGCAGACTGGGCC14480.11255002763227544No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14440.11223911595373323No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAGTCGGCCCTCAGGGGTTTCTAGTCG14430.11216138803409768No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14150.1099850062843023No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14070.1093631829272179No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA13990.10874135957013352No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG13980.10866363165049796No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG13950.10843044789159131No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT13640.10602088238288929No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAGCGACT13450.10454405190981385No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13430.10438859607054275No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTC13290.10330040519564505No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT13250.10298949351710286No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13190.10252312599928956No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCA13180.10244539807965401No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA13090.10174584680293407No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC13040.10135720720475634No Hit
CTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGC13030.10127947928512077No Hit
GTGTTGTTGGTACCAGGAGACAAAGTTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGAGATCGA12910.10034674424949418No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAG12910.10034674424949418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCA350.003824906461.0522570
AAATCGT400.006488655753.42072370
TTAACGG306.5944594E-445.592735
GTGGTAT3550.044.787561
GGTATCA13400.043.4221731
TGGTATC3850.040.4048082
CGGTACC350.001296962639.73853745
GTATCAA34100.037.400281
TACGCAT501.688658E-435.0768249
TAACGGC400.002698608234.22950436
GCTTAAC400.00285949133.8275877
GTTACAC400.002863853733.8170663
TATCAAC37750.033.7722782
TCTCGCG3950.032.4581670
AAAGCGT450.003569482832.3303363
TCAACGC39800.032.287654
ATCAACG39800.032.2026863
CAACGCA40500.031.6473265
AACGCAG40950.031.1368036
AATGGTA4900.030.52612770
Produced by FastQC (version 0.11.5)