FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133420
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48140.42473222635916075No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33710.297418432708087No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACCAC33310.2938892908189374No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28970.2555981013216636No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGGTCCAGGGAGA27570.24324610470963984No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27140.239452277178804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26280.23186462211713224No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25660.22639445218895024No Hit
GACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACCACAGCGTTCAGG24210.21360131284078276No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23560.20786645727091457No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTC23470.2070724003458559No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21610.19066189056131003No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21550.19013251927793756No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21470.18942669090010764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21370.18854440542782022No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19970.17619240881579645No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATAGGGAGGAATTTTGTATCCTGGTACCAGCAC19970.17619240881579645No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19770.1744278378712216No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT19340.17063401034038572No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19190.1693105821319546No Hit
CTCCAATAGTTACTACTACTGGAGCTGGGTCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGGTACATCT19040.16798715392352348No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18620.16428155493991636No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18480.16304635527871397No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17940.15828201372836195No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17360.15316475798909493No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17300.15263538670572252No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGGTCCAGGGA17130.1511355014028339No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16700.14734167387199804No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15900.14028339009369872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15770.1391364189797251No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15570.13737184803515026No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15340.13534259144888922No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15260.13463676307105926No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14960.13198990665419702No Hit
CTCTTACACTGGGAGCACCTTCTACAATCCCTCCCTCAAGAGTCGGGTCACCATCTCTTCTGACACGTCCAGGAA14850.13101939263468088No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCTCTGG14590.1287254504067336No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14430.12731379365107373No Hit
GTCTATTACTGTGCGCATTATGGTGCGGGGAATTACGTTGGGGGGGGGACCTTTGGCTCCTGGGGCCAGGGAACC14320.1263432796315576No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTATTGCGCATCGTGGGATATGAGCCTGAACG13960.1231670519313229No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13660.12052019551446065No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13310.11743219636145472No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13240.11681459653085352No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13150.11602053960579485No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13100.11557939686965114No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13080.11540293977519367No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12880.11363836883061884No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12850.11337368318893261No Hit
ATATGAGCCTGAACGCTGTGGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT12810.11302076900001765No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12250.10807997035520812No Hit
ACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACCACAGCGTTCAGGC12210.10772705616629316No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12190.10755059907183567No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12090.10666831359954829No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12060.10640362795786204No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11940.10534488539111717No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11790.10402145718268602No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATAGGGAGGAATTTTGTATCCTGGTACCAGCACCTCCCGGGAACGGCCCC11780.10393322863545729No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11760.1037567715409998No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11690.10313917171039862No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG11590.1022568862381112No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11500.10146282931305253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)