FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133420
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46330.40876285931075856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43770.38617635122020083No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31370.2767729526565616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28800.254098216018775No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28060.24756930352384815No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27360.24139330521783628No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACCAC25570.2256003952638916No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24330.21466005540752767No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23130.20407262974007867No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGGTCCAGGGAGA23120.20398440119284997No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21980.19392634680877344No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21690.19136771893913995No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21620.19075011910853876No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21420.18898554816396393No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATAGGGAGGAATTTTGTATCCTGGTACCAGCAC19270.17001641050978455No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18770.16560498314834748No Hit
CTCCAATAGTTACTACTACTGGAGCTGGGTCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGGTACATCT18520.16339926946762895No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17960.15845847082281944No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTGGGTCCCTC17760.1566938998782446No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17550.15484110038644103No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17150.1513119584972914No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC17060.15051790157223272No Hit
GACCTAGGACGGTCAGCTGGGTCCCTCCGCCGAATACCACAGCGTTCAGG16890.1490180162693441No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16870.14884155917488665No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16770.1479592737025992No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16390.14460658890790704No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16030.14143036120767236No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15470.13648956256286285No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15430.13613664837394787No Hit
CTCTTACACTGGGAGCACCTTCTACAATCCCTCCCTCAAGAGTCGGGTCACCATCTCTTCTGACACGTCCAGGAA15220.1342838488821443No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15090.13313687776817068No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15020.13251927793756949No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGGTCCAGGGA14330.12643150817878634No Hit
GTCTATTACTGTGCGCATTATGGTGCGGGGAATTACGTTGGGGGGGGGACCTTTGGCTCCTGGGGCCAGGGAACC14150.12484339432866898No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCTCTGG13850.12219653791180675No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13790.1216671666284343No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13730.12113779534506183No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTATTGCGCATCGTGGGATATGAGCCTGAACG13670.12060842406168941No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13660.12052019551446065No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13480.11893208166434333No Hit
ATATGAGCCTGAACGCTGTGGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT13440.11857916747542835No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13430.1184909389281996No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13290.11725573926699723No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13210.1165499108891673No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13090.1154911683224224No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12990.114608882850135No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12840.11328545464170388No Hit
GTGGTATTCGGCGGAGGGACCCAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12690.11196202643327276No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG12680.111873797886044No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGTT12210.10772705616629316No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12150.10719768488292071No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12080.10658008505231953No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11610.10243334333256869No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11540.1018157435019675No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11420.1007570009352226No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11390.10049231529353639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.00415802559.7775670
ACGTCAA305.43667E-447.41346452
GCGATTT658.877791E-442.9172270
GGTATCA14400.041.594331
GTATCAA33850.036.2836341
AGAAGAG11050.034.71245670
TGGTATC4250.033.240262
CCCCGCA1650.032.6051949
AATGGTA4750.032.30085870
ACGTAAT1700.031.669971
TATCAAC38650.031.5038172
ATCAACG40200.030.1151263
TCAACGC40200.030.030154
AACGCAG40950.029.643076
CAACGCA41100.029.3712565
CGTAATT1850.029.0905782
CCGTTAA606.290955E-428.02874816
GCCGTTA606.294203E-428.02627215
ACGCAGA43500.027.9053757
ACGGGGT2300.027.78501716
Produced by FastQC (version 0.11.5)