FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618114
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49970.30881631331290627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47470.2933662275958307No Hit
CATCTATGGTGCGTCCACCAGGGCCTCTGCCATCGCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA38360.23706611524280738No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36200.22371724118325406No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA35050.21661020175339935No Hit
CCTCCTACCTTCGGCCAAGGGACCACGGTGGACATCACACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG32730.20227252220795322No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31190.19275526940623466No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29990.1853392282620384No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCATTGCGCGCAGGCTCAGGTATA29680.18342341763312103No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29150.180147999461101No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28980.17909739363233987No Hit
GTCTTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGA28550.1764399788890029No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28490.17606917683179305No Hit
CCATAGATGAGGAGCCTCGGAGATTGGCCAGGTTTTTGAAGATACCAGGC27790.1717431528310119No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27770.1716195521452753No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27210.16815873294465036No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27000.16686092574441602No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26950.16655192403007452No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGTCCGAGGATT26930.1664283233443379No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26690.16494511511549867No Hit
GGTAGGAGGTGACCAACGATACTGCTGACAGTAAAATACTGCAAAATCCT24550.151719841741682No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCGACAC24280.15005123248423782No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCGACACCAACCTAGCCTGGTATCTTCAAAAACCTGGCCAATCTCCGAGGCT23910.14776461979811062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23770.14689941499795442No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23600.14584880916919327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23090.14269699168290984No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21900.13534275088158187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21900.13534275088158187No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21810.13478654779576715No Hit
GTTTATTACTGTGCGAGACATGAAGGGGGTAGTGGTTGGGACGAGAACTTCTTCGACTACTGGGGCCAGGGAATC21160.13076952550932752No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20940.1294099179662249No Hit
ATTTTACTGTCAGCAGTATCGTTGGTCACCTCCTACCTTCGGCCAAGGGA20920.12928631728048828No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT20260.12520749465118033No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20220.12496029327970712No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGTCCGAGGATTTTGCAGTATTTTACTGTCAGCAGTATCGTTGGTCA20220.12496029327970712No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20160.12458949122249731No Hit
TCTATGGTGCGTCCACCAGGGCCTCTGCCATCGCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19970.12341528470799956No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC19300.11927466173582331No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19250.1189656600214818No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19240.1189038596786135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19220.11878025899287689No Hit
GTATTTTACTGTCAGCAGTATCGTTGGTCACCTCCTACCTTCGGCCAAGGGACCACGGTGGACATCACACGAA19030.11760605247837916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18850.11649364630674972No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGCGATGGCAGAGGCCCTGGT18810.1162464449352765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATGTCCACCGTGGTCC18670.11538124013512026No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18590.11488683739217385No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18360.11346542950620289No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17740.10963380824836816No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG17270.10672919213355796No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17240.10654379110495306No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16620.10271216984711833No Hit
GTGCAGCCACAGTTCGTGTGATGTCCACCGTGGTCCCTTGGCCGAAGGTA16590.10252676881851341No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16450.10166156401835717No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16420.10147616298975227No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16410.10141436264688396No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16390.10129076196114736No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16290.10067275853246434No Hit
GCCACAGTTCGTGTGATGTCCACCGTGGTCCCTTGGCCGAAGGTAGGAGG16240.10036375681812283No Hit
GTAAAATACTGCAAAATCCTCGGACTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16210.10017835578951793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)