FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618114
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54830.3388512799469012No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48300.2984956560538998No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39170.24207194301513985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36730.2269926593552741No Hit
CATCTATGGTGCGTCCACCAGGGCCTCTGCCATCGCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA35930.22204863192580993No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA33630.2078345530661004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29170.18027160014683763No Hit
CCTCCTACCTTCGGCCAAGGGACCACGGTGGACATCACACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG29170.18027160014683763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28880.17847939020365686No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCATTGCGCGCAGGCTCAGGTATA28640.1769961819748176No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28540.17637817854613458No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGTCCGAGGATT27460.16970374151635792No Hit
GTCTTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGA27000.16686092574441602No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26970.16667552471581112No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25690.15876508082866844No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25550.15789987602851221No Hit
CCATAGATGAGGAGCCTCGGAGATTGGCCAGGTTTTTGAAGATACCAGGC24830.15345025134199444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24820.15338845099912612No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCGACACCAACCTAGCCTGGTATCTTCAAAAACCTGGCCAATCTCCGAGGCT23890.14764101911237404No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23150.1430677937401197No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22680.14016317762530947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22670.14010137728244115No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22610.13973057522523136No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCGACAC22300.13781476459631398No Hit
GGTAGGAGGTGACCAACGATACTGCTGACAGTAAAATACTGCAAAATCCT22190.13713496082476265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21510.13293253750971812No Hit
GTTTATTACTGTGCGAGACATGAAGGGGGTAGTGGTTGGGACGAGAACTTCTTCGACTACTGGGGCCAGGGAATC21440.13249993510963998No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGTCCGAGGATTTTGCAGTATTTTACTGTCAGCAGTATCGTTGGTCA21040.1300279213949079No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20970.1295953189948298No Hit
ATTTTACTGTCAGCAGTATCGTTGGTCACCTCCTACCTTCGGCCAAGGGA20740.1281739111088588No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20350.12576369773699506No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20170.1246512915653656No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19760.12211747750776522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19590.12106687167900408No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19540.12075786996466258No Hit
TCTATGGTGCGTCCACCAGGGCCTCTGCCATCGCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19130.11822405590706218No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19010.11748245179264254No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18770.11599924356380328No Hit
GTATTTTACTGTCAGCAGTATCGTTGGTCACCTCCTACCTTCGGCCAAGGGACCACGGTGGACATCACACGAA18640.11519583910651536No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18510.11439243464922742No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18510.11439243464922742No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGCGATGGCAGAGGCCCTGGT18270.11290922642038818No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18170.11229122299170516No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17800.11000461030557798No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATGTCCACCGTGGTCC17640.10901580481968513No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17590.10870680310534364No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16600.1025885691613817No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG16420.10147616298975227No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16390.10129076196114736No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16280.10061095818959603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)