FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508933
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT100680.6672264441164717No Hit
GATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCA99870.6618584125338899No Hit
GTATTAAGTTGCTGACAGTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTC95680.6340904466931269No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91650.6073828327699109No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91320.6051958569399701No Hit
GTATAGATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGC85380.5658302920010365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC75730.5018778169739809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65250.4324247663746502No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59790.39624025718835765No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA58090.38497401806442033No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56320.37324387497655626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55600.3684722913475946No Hit
GGGTAAGTATTAAGTTGCTGACAGTAATAAGTGGCAAAATCATCAGGCTG55050.3648273316310267No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG53290.3531634605380093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52890.3505125807441417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT51170.3391137976305111No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG49810.3301008063313613No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49440.3276487425220338No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45980.3047186323050791No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45460.3012724885730513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC45020.29835652079979696No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC44140.2925245852532882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41950.2780110183818632No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG41390.2742997866704486No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGT40850.27072109894872737No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39730.2632986355258981No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39640.26270218757227787No Hit
GTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAAGTGCTA39400.26111165969595734No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCACCGCCGAAAGTGAGCTCAGGGTAAGTATTAAGTTG39150.2594548598247901No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT38650.25614126008245564No Hit
TACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT37070.24567028489667864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35750.23692238157691559No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC35440.23486794973666825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33610.22274017467972404No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTC32440.21498635128266133No Hit
CCCCCTAGACTCCTGATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCT31910.21147393555578678No Hit
GACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTATTACTGTCAGCAACTTAATACTTAC31670.20988340767946623No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31600.20941950371553938No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31590.2093532317206927No Hit
CAGTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCG30300.20080414438546973No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29670.19662900871012826No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGAT29300.19417694490080079No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC28870.1913272491223931No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA28840.19112843313785305No Hit
CTCCTGATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGA28460.18861009733367884No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27830.1844349616583374No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27550.18257934580263008No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC27200.18025982598299592No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT27100.17959710603452902No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT27080.17946456204483566No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27040.1791994740654489No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26970.17873557010152208No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26690.17687995424581476No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG26570.1760846903076545No Hit
GATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT26270.1740965304622538No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26150.17330126652409353No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC26030.17250600258593324No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26000.17230718660139316No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25690.1702527547611458No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25320.16780069095181827No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT24760.16408945924040366No Hit
AGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATA24660.16342673929193677No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24410.1617699394207695No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24300.16104094747745593No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24010.15911905962690193No Hit
ATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACTCTCA23990.15898651563720853No Hit
CCTTGGTCCCACCGCCGAAAGTGAGCTCAGGGTAAGTATTAAGTTGCTGA23940.1586551556629751No Hit
GTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCA23400.15507646794125385No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23340.15467883597217372No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22970.1522267721628462No Hit
GGCTAAATAAGTGCTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGAA22720.15056997229167896No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22600.1497747083535187No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG22550.14944334837928522No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC22490.1490457164102051No Hit
GTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCG22440.14871435643597164No Hit
ACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCAT22270.14758773252357793No Hit
CATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATTTTG22240.14738891653903785No Hit
CATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTGCTTCCA22170.146925012575111No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTGGAAGCAGTATAGATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTG21730.14400904480185667No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21700.1438102288173166No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21700.1438102288173166No Hit
CTTATTACTGTCAGCAACTTAATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAA21030.1393700051625884No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20800.13784574928111454No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20550.1361889494099473No Hit
CCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCG20450.1355262294614804No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20370.13499605350270688No Hit
ATCTCGGACAGACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATT20290.13446587754393338No Hit
ATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAA20280.13439960554908667No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20240.1341345175696999No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20180.13373688560061978No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAA20160.13360434161092638No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19840.13148363777583233No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19710.13062210184282538No Hit
GTGCTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGAACGATGGAGA19550.12956174992527833No Hit
CTCACAATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTATTACTGTCA19500.12923038995104488No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19440.12883275798196472No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19260.12763986207472433No Hit
GGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTGCTTC19070.12638069417263723No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTC19020.12604933419840378No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG18800.1245913503117766No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18710.1239949023581564No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18540.12286827844576267No Hit
GATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCGCTGAACCTTGATG18360.12167538253852225No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG17970.11909077473950136No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17870.11842805479103445No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAG17780.11783160683741425No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGA16900.11199967129090556No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG16820.11146949533213203No Hit
ACTTATTACTGTCAGCAACTTAATACTTACCCTGAGCTCACTTTCGGCGG16810.11140322333728536No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16610.11007778344035155No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16600.11001151144550488No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16560.10974642346611811No Hit
GTCTCCCACAGAAGCAGACAGGAACGATGGAGACTGGGTCAAGCGGACGTCACATCTGGCACCTGGGAGCCAGAG16530.10954760748157805No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16480.10921624750734459No Hit
GCCACAGTTCGTTTGATGTCCACCTTGGTCCCACCGCCGAAAGTGAGCTC16420.10881861553826445No Hit
GACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16380.10855352755887769No Hit
GTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCA16290.1079570796052575No Hit
GTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTG16170.1071618156670972No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16050.10636655172893694No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15880.10523992781654322No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15850.10504111183200315No Hit
GAGTGAAGTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15700.1040470319093028No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCC15590.1033180399659892No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACT15580.10325176797114254No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAC15500.10272159201236901No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC15430.10225768804844218No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA15380.10192632807420872No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT15350.10172751208966865No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT15250.10106479214120176No Hit
CCCCTAGACTCCTGATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCA15170.10053461618242826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT3850.046.79157326
GCGTAGT3900.046.25465429
CGTATGC3800.045.4022141
ACGCGTA4000.045.0548427
CGCGTAG4050.044.52375428
AATCTCG3700.044.44353536
TCGTATG3900.044.17402640
AGACGCG4150.043.39463825
CGTAGTA4250.042.48782730
ATCTCGT4050.042.327137
ATGCCGT4100.042.29537244
TATACAC4350.042.1176953
CGAGACG4300.041.851723
ACGAGAC4300.041.8461522
CTTATAC4250.041.5120851
GTAGTAA4400.041.0831331
GTATGCC4300.041.0013542
TAGTAAT4450.040.65267632
GTAATCT4050.040.5173134
CACGAGA4450.040.4262221
Produced by FastQC (version 0.11.5)