FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508933
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCA97060.6432359819819701No Hit
GTATTAAGTTGCTGACAGTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTC94590.6268667992548377No Hit
ACTTAATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT93970.6227579355743429No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89640.5940621618057262No Hit
GTATAGATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGC84290.5586066445627473No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81890.5427013657995419No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC72710.4818636745302807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68520.45409570868951765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC61230.4057834244462809No Hit
GGGTAAGTATTAAGTTGCTGACAGTAATAAGTGGCAAAATCATCAGGCTG56150.37211725106416255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA54970.3642971556722532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53920.35733859621335073No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49970.33116115824890835No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49190.3259919426508665No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48390.32069018306313135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG47330.31366535160938225No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47020.31161091976913485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT46670.3092913999495007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44770.2966997209286297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44570.2953742810316959No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44500.29491037706776907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT41710.2764204905055427No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC40620.2691968430672535No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCACCGCCGAAAGTGAGCTCAGGGTAAGTATTAAGTTG40310.2671424112270061No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39140.25938858782994345No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT38360.2542193722319016No Hit
GTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAAGTGCTA38110.25256257236073437No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37340.24745962875753927No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA36510.241959053185264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36400.24123006124195046No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGT35700.23659102160268217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35150.23294606188611422No Hit
TACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT34580.2291685581798529No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34230.2268490383602188No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA32330.21425735933934772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32130.21293191944241394No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31760.21047985563308644No Hit
GACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTATTACTGTCAGCAACTTAATACTTAC31710.21014849565885296No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC30970.20524436804019794No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTC30500.20212958428240352No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30440.2017319523133234No Hit
CAGTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCG29720.19696036868436176No Hit
CTCCTGATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGA28620.18967044925122586No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC28170.18668820948312484No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27800.1842361456737973No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27600.1829107057768635No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA27430.1817840818644698No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGAT27210.18032609797784263No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26980.17880184209636876No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAA26690.17687995424581476No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC26600.17628350629219455No Hit
CCCCCTAGACTCCTGATCTATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCT26590.17621723429734787No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26540.1758858743231144No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC25870.1714456506683862No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25310.16773441895697158No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25170.16680661102911792No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC25110.1664089790600378No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25050.16601134709095766No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24940.16528235514764406No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24810.16442081921463708No Hit
GATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT24260.16077585949806916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23340.15467883597217372No Hit
GTAATAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCG23050.15275694812161972No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23000.15242558814738627No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC22860.1514977802195326No Hit
AGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATA22830.15129896423499253No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22740.15070251628137235No Hit
GTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCA22680.15030488431229222No Hit
GGCTAAATAAGTGCTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGAA22430.14864808444112496No Hit
ATACTGCTTCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACTCTCA22430.14864808444112496No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21910.1452019407090971No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21890.14506939671940372No Hit
GTGCTAATGTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAGACAGGAACGATGGAGA21740.14407531679670335No Hit
CCTTGGTCCCACCGCCGAAAGTGAGCTCAGGGTAAGTATTAAGTTGCTGA21690.14374395682246993No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21560.14288242088946296No Hit
CATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTGCTTCCA21250.14082798904921556No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACT21170.14029781309044206No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTGGAAGCAGTATAGATCAGGAGTCTAGGGGGTTTCCCTGGTTTTTG21150.14016526910074867No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT21140.14009899710590198No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21120.1399664531162086No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21080.13970136513682183No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC20410.13526114148209364No Hit
GTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGT20230.13406824557485322No Hit
CTTATTACTGTCAGCAACTTAATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAACGAA20170.1336706136057731No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20000.13254398969337935No Hit
CATCAAGGTTCAGCGGCAGTGGATCTCGGACAGACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATTTTG19870.1316824537603724No Hit
ATCTCGGACAGACTTCACTCTCACAATCAGCAGCCTGCAGCCTGATGATT19790.13115227780159888No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19740.13082091782736543No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG19680.13042328585828528No Hit
GAGTGAAGTCTGTCCGAGATCCACTGCCGCTGAACCTTGATGGGACCCCA19580.12976056590981838No Hit
CCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCACTTGCCG19450.12889902997681144No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19200.1272422301056442No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19130.12677832614171736No Hit
ATACTTACCCTGAGCTCACTTTCGGCGGTGGGACCAAGGTGGACATCAAA19100.12657951015717728No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19100.12657951015717728No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19090.1265132381623306No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18700.1239286303633097No Hit
GGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTGCTTC18580.12313336642514944No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTC18560.12300082243545604No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18550.12293455044060937No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18500.12260319046637591No Hit
GATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCCGCTGAACCTTGATG18440.12220555849729578No Hit
ACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCAT18090.11988603867766164No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG17970.11909077473950136No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17860.11836178279618778No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGAAGCAG17760.11769906284772089No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT17520.11610853497140032No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17470.11577717499716687No Hit
GGCTGCAGGCTGCTGATTGTGAGAGTGAAGTCTGTCCGAGATCCACTGCC16620.11014405543519824No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16450.10901743152280452No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16360.10842098356918432No Hit
CTCACAATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTATTACTGTCA16300.10802335160010416No Hit
GTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGACAGAGTCACCATCA16150.10702927167740384No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA16060.10643282372378361No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15910.10543874380108328No Hit
GTCTCCCACAGAAGCAGACAGGAACGATGGAGACTGGGTCAAGCGGACGTCACATCTGGCACCTGGGAGCCAGAG15880.10523992781654322No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCC15810.10477602385261639No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC15760.10444466387838293No Hit
ACTTATTACTGTCAGCAACTTAATACTTACCCTGAGCTCACTTTCGGCGG15580.10325176797114254No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACGTCCGCTTGACCCAGTCTCCATCGTTCCTGTCTGCTTCTGTGGGAGA15560.10311922398144914No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15460.10245650403298225No Hit
GTCAGGACATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAACCCCCTAGACTCCTGATCTATACTG15390.10199260006905542No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15220.1008659761566617No Hit
GCCACAGTTCGTTTGATGTCCACCTTGGTCCCACCGCCGAAAGTGAGCTC15200.10073343216696833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA200.00792795450.7851031
GTGGTAT3900.042.5379451
CGATTGC457.4180534E-540.33830363
GTATCAA29900.037.0272671
GATTCAC3350.033.351411
TATCAAC34050.032.607342
ATCAACG35200.031.7312223
CAACGCA35150.031.5827275
AACGCAG36200.030.677846
TGTTAGA30750.030.6395370
ACGCAGA36950.029.8778277
TAGATTA703.6313624E-529.82784846
ATTCACT3800.029.3960552
CGCAGAG37650.029.2382018
TCAACGC38100.029.226184
TAATACC704.333146E-529.06534420
GCCTGAC9450.029.0267947
AGAGTAC37850.029.00672111
CAGAGTA38050.028.94139110
GCAGAGT39000.028.3185969
Produced by FastQC (version 0.11.5)