FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199491
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38710.322720220493526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34950.29137359096483423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33920.282786615322666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29250.24385343449846641No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28790.24001847450293498No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26390.22000998756972748No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25110.20933879453868348No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22440.18707935282549015No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22330.1861622971743848No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCA22320.18607892847882976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22020.1835778676121788No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21330.17782542761888168No Hit
ACTTATTACTGTCAACAGGTTAAATATTACCCTATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20710.17265656849446973No Hit
GGGTAATATTTAACCTGTTGACAGTAATAAGTTGCGAGATCTTCAGGCTG20570.1714894067566993No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20430.17032224501892884No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20400.17007213893226375No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20150.16798792154338799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19920.16607044154562225No Hit
GGTTAAATATTACCCTATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19840.165403491981182No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19270.16065147633454524No Hit
GTGCGGTCCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCACT19000.1584005215545594No Hit
ATATTACCCTATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT18480.15406534938569777No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18170.1514809198234918No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTTGGAGACAGAGTCACCGTCACTT18070.1506472328679415No Hit
GTAATAGATAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA18010.1501470206946113No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17060.14222699461688332No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16960.141393307661333No Hit
GTTGGAGACAGAGTCACCGTCACTTGCCGGGCCAGTCAGGGCATTGACAGTCATTTAGCCTGGTATCAGCAAAAA16920.1410598328791129No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16690.13914235288134716No Hit
GTAATAAGTTGCGAGATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAAATCCACTGCCG15900.1325562259324997No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15780.13155580158583932No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15450.12880463463252328No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15220.12688715463475755No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15150.12630357376587237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15150.12630357376587237No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14870.12396925029033148No Hit
GGGCATTGACAGTCATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC14850.12380251289922142No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14680.12238524507478589No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATCTCGCAACTTATTACTGTCAACAGGTTAAATATTAC14300.11921723464369471No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13710.11429848160594785No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13660.1138816381281727No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13640.11371490073706263No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13330.11113047117485665No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13030.10862941030820572No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13020.1085460416126507No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12770.10646182422377493No Hit
CATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGAT12720.10604498074599977No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12670.1056281372682246No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12550.10462771292156423No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12520.10437760683489913No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12370.10312707640157366No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCAAGGTT12270.10229338944602334No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12250.1021266520549133No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12180.10154307118602807No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12170.10145970249047305No Hit
TATTTAACCTGTTGACAGTAATAAGTTGCGAGATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC12150.10129296509936297No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC12030.10029254075270261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)