FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length33-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG22.2222222222222223No Hit
GCTTATATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC11.1111111111111112No Hit
GCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCA11.1111111111111112No Hit
AAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAG11.1111111111111112No Hit
ACAGTGACGTTGGTTATTATAATTTTGTCTCCTGGTACCAACAGTACCCA11.1111111111111112No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT11.1111111111111112No Hit
GAACAGGGCCACCACCTCCCACGGCGGCGCCACCTCCTGCACCTCCGGCCCGAAAGACTTACCGCATAATCACA11.1111111111111112No Hit
TGACTTACCAGTCAGATTATTCAAAAATATATGAAAACCAAAAATCAGAAACAACCCAAAGACCATAAAATACTA11.1111111111111112No Hit
ACAGGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCTTGACGCTGAGCAAAG11.1111111111111112No Hit
CAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11.1111111111111112No Hit
CAAATAAACATCACCGATCACAACATACGGAAACATATCAACTATTACCAATGTTCCATATAATAAAAAAAGATG11.1111111111111112No Hit
AAATTGGGAAGAGCGCTGTTAAAGGCCACCCTGCTCGTGACCTCTGTCGCGGCTACCTCTTGTACAGTGTCATGC11.1111111111111112No Hit
GCTTGCCCTGAGTACTGGCTTCTGCTTTCTGCTTCTGAGGCACCATGCATTTAGATGCAGAAGGCAGGGCGGCT11.1111111111111112No Hit
CAACAGAGGCAGTTCCAGATATCAACTGCTCATCACATGGCGGGAAGATGAAGACAGAAGGTGCAGCCACAGTT11.1111111111111112No Hit
AATCCACTCCAGTCCCTTCCCGGCGGACTGCCGGATCCAGCTCCAATAGT11.1111111111111112No Hit
ACAATATTTTTCTGACAAAGTGAAAAAAGTGAATAATCATGAAAAGAAAG11.1111111111111112No Hit
CTTATTACTGCCAACAACATGATAATTATGTGACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGG11.1111111111111112No Hit
ACCTTTATTTACTAGCAATCAATATGGTAAATTAGTTCCCTAACATTCTC11.1111111111111112No Hit
GACAATGAGTTCTTGGCATTGTCTCTGGAGATGGTGGATCGGCCCTTCACTGAGTCTGCGTAGACTGTGTAAGTC11.1111111111111112No Hit
AAATTACAGCATGCAAGCACTACAAACTCCTCGGACGTACGGCCAAGGCACCAAGCAGGAAATCAAACGAA11.1111111111111112No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGATCAGCTCCTGGGGCTC11.1111111111111112No Hit
GAGTACTGTTTTAGTTTTTTTTTTTTTATTTTTTTAATGTTTCAAACAACACAAAGTCGAAGCAACCAAACACA11.1111111111111112No Hit
CAGTAGCACTGTCTTCATCACACATATTCCAATGGGTAGCACTAACTATC11.1111111111111112No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTG11.1111111111111112No Hit
GACCATGAATAACTACTCTGAGAAACAATCCTCCTAGTAACACCAATCCAGTGCGAAAAAAAACATACACAGA11.1111111111111112No Hit
CAGATGTCCTGGTTGCCAGTGACTTTTACCACTGTCTGATAGGTAATGAC11.1111111111111112No Hit
ACAGGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTG11.1111111111111112No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTAACTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11.1111111111111112No Hit
CTTATGACCCTGTCTCCTGGTCCCAACAGCACCCATGATTTACAATGTCA11.1111111111111112No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAG11.1111111111111112No Hit
CACACAGAGCGGCCAGCCGCCCAGAGCCTGTGGGCACGCCAGCACGCACA11.1111111111111112No Hit
CAGATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAG11.1111111111111112No Hit
GACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGC11.1111111111111112No Hit
AGGCACACAACAGAGGCAGTTCCAGATTTCAAC11.1111111111111112No Hit
GAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGTAGGAGTTTATCACCAGG11.1111111111111112No Hit
GTGTTGTTGGAACCAGGAGACATAGTCAAAACCACCAACGTCACCGCTGCTTCCAGTGCAGGAGATGGTGATCGA11.1111111111111112No Hit
GTTTTACATCACCCAAAAAAAAAAAAAAAACCCCGGGTTTCAAATTCATTGGAAATAAATATCATAAATTTGTGA11.1111111111111112No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGA11.1111111111111112No Hit
TCCGACGGCGGCGGCAGCAGGTGGACCTCGGGCCGGAATGTGTTTCCGGA11.1111111111111112No Hit
ACATTACTGTCACCAGTAAATTCAAGACTTTCCAATTTCATTTCAGTGGAACATCAGAACTTCCAGCTTCTGTG11.1111111111111112No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATGTATTTGGTTTCTAAT11.1111111111111112No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11.1111111111111112No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11.1111111111111112No Hit
GCGCCGTCGGAGGGGCCGCGGGGCTGCAGTTCAGGAGGCCGGAGAAGCGC11.1111111111111112No Hit
CAGTATAGTAGTTATTCTGCATTCAAATTCGGCCCCGGGACCAAAGTGGA11.1111111111111112No Hit
CATTTATGTGATGATGAAAGTTATTTAGCATCTTTATGGAAGGACTTAGC11.1111111111111112No Hit
GAACGAGCTGGTGACGCTGACGTGCCTGGCACGCGGCTTCAGCCCCAAGG11.1111111111111112No Hit
GTATCAACGCAGAGTACGGGAGAAGACCATCGACCGCTTGGCCGGTAAACCCACCCATGTCAATGTGTCAGT11.1111111111111112No Hit
AAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTGTATGAATGGCTC11.1111111111111112No Hit
CTAACCGACCAATCCTATAACATGACTCACCCACCCTCAGTCAAAGTTACAAAAGGACACACATCCACCAT11.1111111111111112No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATAGGTACTACTATT11.1111111111111112No Hit
ACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAA11.1111111111111112No Hit
ACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAG11.1111111111111112No Hit
GACAATCATAACACCAAAATAACATAAACCTACAACAATAACAGCATGATCCCCAACTCCCCCCCCAACAGA11.1111111111111112No Hit
CTCTTCATCATCCCCACATCTGAAACCAAGAAGAAATATGTAACACCCTAGTTACATTCCAATAAGAAAAAATT11.1111111111111112No Hit
GGAGGCCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCC11.1111111111111112No Hit
GTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCC11.1111111111111112No Hit
AGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACACCTCCCCTGGGATCCTG11.1111111111111112No Hit
GTCCAAGTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCA11.1111111111111112No Hit
GCTTCACGTGGCATGTCACCGGCTTGCCGGCCCCCCACTGGCGAGCCGGGAAATGGGGGCCAAGCCCCGGGGAG11.1111111111111112No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCTAACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAA11.1111111111111112No Hit
CGGTCTCACCATACTGCCTGGGCAGGTTTCCAACTCCTGGGCTCAAGTGA11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11.1111111111111112No Hit
GCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTG11.1111111111111112No Hit
AGTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAA11.1111111111111112No Hit
AAGCTGGCCCACATCAAGAGGCTCATCGCCGAGTACGAACAGCGGCAGCTGCAGGCTTGGCCCTAGCCGCCCTCC11.1111111111111112No Hit
CTGCATTGTTGACCCAGTGTCAGAAAATGGACATCCCTTTCCTTGGGG11.1111111111111112No Hit
GTACCAGACCCTGCAGCCGCAGAGATGTTGATGCCTAAGAAGAACCGGAT11.1111111111111112No Hit
ACACAAAATACTATATAAATATAAATTTTTATGAAAAATATATATAAATAATCCCCCCAAATTATAATAATTTTA11.1111111111111112No Hit
GACCAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGAC11.1111111111111112No Hit
AACTACAACATAAAAACCAAGACACCAAAGACCAGATCATCAAACCAAAAACAGATAAAATTACAACCAAAAATA11.1111111111111112No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGAGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGACGAGCCA11.1111111111111112No Hit
GTAGTTATTGTTAAAATCAGCTGCACTAGATACAATTTGAAAATATCCAGCACCAGGTTAATTCCAATAATGAAC11.1111111111111112No Hit
AGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAG11.1111111111111112No Hit
GCCCAGTTCCCTCAACTCAACCTACCCCATCTCCCTCAACTCAACCTACACCATCTCCCACAAGCTGCCACCCC11.1111111111111112No Hit
GAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTC11.1111111111111112No Hit
AACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACACGGGAGCCGT11.1111111111111112No Hit
GGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGA11.1111111111111112No Hit
TTATGAATCCTGGTTCCAGCAGCTCCCACGAACGGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCT11.1111111111111112No Hit
ATATATTGTTAGGAGAATTCAAGTTATTGAAACTTGCCAAATTCTTGAGT11.1111111111111112No Hit
GTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTT11.1111111111111112No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11.1111111111111112No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTCACGGGCGAGCTC11.1111111111111112No Hit
GTGTCTGATCAGTGACTTATACCCGGGAGCTGTGACAGTGTAATGGAAGG11.1111111111111112No Hit
GCATAATGGTGATCACACGTTCCACCTCATCCTCAGTGAGTTCTCCCGCC11.1111111111111112No Hit
ACTCAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAG11.1111111111111112No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11.1111111111111112No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC11.1111111111111112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)