FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562008
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29200.5195655577856543No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGCCACGCAGCGTACTGTCATAGGA23970.426506384250758No Hit
GTCATAGGACTGGCAGAAATAATCGGCCTCATCCTCAGCCTGGAGCCCAG22790.4055102418470911No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21440.3814892314700146No Hit
GTCCTATGACAGTACGCTGCGTGGCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG21040.37437189506199203No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19930.35462128652972913No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGCCACGCAGC19240.34234388122589005No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19100.3398528134830821No Hit
CTCCTGTAGTGGGAGCAACTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAACTTCCACGAACAGC18920.33665001209947193No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16780.29857226231655065No Hit
ATCGTAATAGTCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGGCTGGCACCTCAGCCTCCCTGG16570.29483566070233874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15780.280778921296494No Hit
CTATTACGATAGATGAGGAGTTTGGGGGCTGTTCGTGGAAGTTGCTGGTA15090.2685015159926549No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15050.2677897823518527No Hit
GGTTATGATGTCCACTGGTACCAGCAACTTCCACGAACAGCCCCCAAACTCCTCATCTATCGTAATAGTCATCGG14910.2652987146090447No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14600.25978277889282714No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT14360.25551237704801355No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12810.22793269846692576No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12800.22775476505672515No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12770.2272209648261235No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCCGATTATTTCTGCCAGT12710.2261533643649201No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12620.22455196367311497No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGCCCTGGAGCCAGAGAATCGGTC12430.2211712288793042No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12320.219213961367098No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12230.2176125606752929No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12190.21690082703449062No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12170.21654496021408948No Hit
CTACAGGAGATGCTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG12010.21369802565088045No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11820.21031729085706966No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11550.20551308878165434No Hit
GCGTACTGTCATAGGACTGGCAGAAATAATCGGCCTCATCCTCAGCCTGG10950.19483708416962037No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10830.19270188324721357No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10560.18789768117179825No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGCCACGCAGCG10440.18576248024939146No Hit
CTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAACTTCCAC10400.1850507466085892No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10390.18487281319838864No Hit
GTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAGCATCTC10240.18220381204538014No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10220.181847945224979No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10110.17989067771277276No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC9690.172417474484349No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9610.17099400720274444No Hit
ACAGTACGCTGCGTGGCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT9540.16974847333134047No Hit
GTTTGGGGGCTGTTCGTGGAAGTTGCTGGTACCAGTGGACATCATAACCTGCCCCGATGTTGGAGTTGCTCCCA9500.16903673969053823No Hit
GTCATAGGACTGGCAGAAATAATCGGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGC9470.16850293945993652No Hit
GCCCCCAAACTCCTCATCTATCGTAATAGTCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGG9300.16547807148652688No Hit
CCTGTAGTGGGAGCAACTCCAACATCGGGGCAGGTTATGATGTCCACTGGTACCAGCAACTTCCACGAACAGCCC9260.16476633784572461No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCCGATTATTTCTGCCAGTCCTAT9180.16334287056412009No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGC9020.16049593600091103No Hit
CATCATAACCTGCCCCGATGTTGGAGTTGCTCCCACTACAGGAGATGCTGACCGTCTGCCCTGGGGCCCCAGACA8850.15747106802750138No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8680.15444620005409174No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8650.15391239982349006No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8510.15142133208068215No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8360.14875233092767362No Hit
ATGTTGGAGTTGCTCCCACTACAGGAGATGCTGACCGTCTGCCCTGGGGC8210.14608332977466512No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8140.14483779590326115No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7970.14181292792985153No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCAGCATCTCCTGTAGTGGGAGCAA7940.14127912769924983No Hit
CCCCTGAGGGCCGATGACTATTACGATAGATGAGGAGTTTGGGGGCTGTTCGTGGAAGTTGCTGGTACCAGTGGA7910.14074532746864815No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7870.14003359382784586No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGGCTGGCACCTCAG7820.13914392677684304No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGCCCTGGAGCC7730.13754252608503792No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7700.13700872585443624No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7670.13647492562383454No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7640.13594112539323283No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGCG7630.13576319198303227No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7620.1355852585728317No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC7590.13505145834223No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAG7540.13416179129122716No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7530.1339838578810266No Hit
GTTATGATGTCCACTGGTACCAGCAACTTCCACGAACAGCCCCCAAACTCCTCATCTATCGTAATAGTCATCGGC7530.1339838578810266No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7510.13362799106062548No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7410.1318486569586198No Hit
TCATAACCTGCCCCGATGTTGGAGTTGCTCCCACTACAGGAGATGCTGACCGTCTGCCCTGGGGCCCCAGACA7290.129713456036213No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGCCCTGGAGCCAGAGAATCGGTCAGGGACC7210.12828998875460848No Hit
GGATGAGGCCGATTATTTCTGCCAGTCCTATGACAGTACGCTGCGTGGCGTGCTATTCGGCGGAGGGACCAAG7130.12686652147300395No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7130.12686652147300395No Hit
GCTCCAGGCTGAGGATGAGGCCGATTATTTCTGCCAGTCCTATGACAGTA6940.12348578667919319No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6760.12028298529558297No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6760.12028298529558297No Hit
GGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCT6740.11992711847518185No Hit
CCTGAGGGCCGATGACTATTACGATAGATGAGGAGTTTGGGGGCTGTTCG6680.11885951801397845No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC6640.11814778437317618No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT6570.1169022505017722No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGGCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG6530.11619051686096996No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6530.11619051686096996No Hit
GCTTGGTCCCTCCGCCGAATAGCACGCCACGCAGCGTACTGTCATAGGACTGGCAGAAATAATCGGCCTCATCCT6520.11601258345076938No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6480.11530084980996712No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC6420.11423324934876372No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6390.11369944911816202No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC6260.11138631478555465No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6260.11138631478555465No Hit
GCACAGGGTCCTGGGCCCAGTCTGCGCTGACGCAGCCGCCCTCAGTGTCT6250.11120838137535409No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC6210.11049664773455181No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC6160.109606980683549No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6160.109606980683549No Hit
GAATAGCACGCCACGCAGCGTACTGTCATAGGACTGGCAGAAATAATCGG6140.10925111386314784No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6100.10853938022234559No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6090.10836144681214502No Hit
CTCCAGGGCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTG6050.10764971317134275No Hit
GGCTGAGGATGAGGCCGATTATTTCTGCCAGTCCTATGACAGTACGCTGCGTGGCGTGCTATTCGGCGGAGGGAC6030.10729384635094162No Hit
TCCTATGACAGTACGCTGCGTGGCGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG5910.10515864542853483No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT5910.10515864542853483No Hit
GCCCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATGACTATTACGATAGATGAGGAGTTTGGGGG5890.1048027786081337No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGCG5870.10444691178773256No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG5820.10355724473672973No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAGCATCTCCTGTAGTGG5790.10302344450612802No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5760.10248964427552631No Hit
TCGTAATAGTCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGGCTGGCACCTCAGCCTCCCTGGC5640.10035444335311952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)