FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946390
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39600.41843214742336665No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37070.39169898244909607No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGATGCAAGTCCAGCCAGAGTCTTTTGTTCAGGTCCAGCAACAACGATTA27170.2870909455932544No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26130.27610181848920634No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25770.2722978898762667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23500.24831200667800804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22020.23267363349147815No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21710.2293980282970023No Hit
GTAATAGACGGCCGTGTCGTCGACTCTCAGGCTGTTCATGTGCAAACTCA21700.2292923636133095No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21590.22813005209268905No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21170.22369213537759275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20760.21935988334618922No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20100.21238601422246645No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCT20020.21154069675292428No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19710.2082650915584484No Hit
GTTTATTACTGTCAGCAATATTATAATAGTCCCCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAA19430.20530648041505087No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAACCCAAGTAGTTGTAA19420.20520081573135812No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18790.1985439406587136No Hit
ATATTATAATAGTCCCCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18640.19695897040332208No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18390.19431735331100286No Hit
GTAATAAACTGCCACATCTTCGGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18010.19030209533067763No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17590.1858641786155813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16880.1783619860733947No Hit
ATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCGGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16760.17709400986908147No Hit
GTCTATTACTGTGTGAAAGATCGGGGTTATGGTTCGACGATCTATTATAATTTCCATGTTTTTGATCTTTGGGGC16760.17709400986908147No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAGATGCAAGTCCAGC16660.17603736303215375No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAGATGCAAGTCCAGCCAGAGTCTTTTGTT16060.1696974820105876No Hit
GTCAGGGACCCCGGACTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGATA15950.16853517048996713No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15510.16388592440748528No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAG15300.16166696604993713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15170.16029332516193112No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14740.15574974376314205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14610.15437610287513606No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14440.15257980325235895No Hit
ACCCGGGAGTCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC13680.1445492872917085No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13680.1445492872917085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13620.14391529918955187No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCCGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATAGT13540.14306998172000973No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12450.13155253119749785No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12340.13039021967687742No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12210.1290165787888714No Hit
CTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCGGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12000.12679762043132325No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11810.12478999144116061No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11790.12457866207377508No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11680.12341635055315463No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11240.11876710447067276No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11160.11792178700113061No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11150.11781612231743784No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11140.11771045763374506No Hit
CTTTTGTTCAGGTCCAGCAACAACGATTACAACTACTTGGGTTGGTATCA10990.11612548737835353No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10800.11411785838819093No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10780.11390652902080539No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10740.11348387028603431No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10700.11306121155126322No Hit
GGACTGAACAGAGAGAACTCACCATGGAGCTTGGGCTGACCTGGCTTCTT10690.11295554686757045No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG10600.11200456471433552No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10320.10904595357093799No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10310.10894028888724522No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10140.10714398926446815No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10020.1058760130601549No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9990.10555901900907659No Hit
CAGTAATAAACTGCCACATCTTCGGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9950.10513636027430552No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9950.10513636027430552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9900.10460803685584166No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGACTCCCGGGT9860.10418537812107058No Hit
GTCCTGGTTTCTGCTGATACCAACCCAAGTAGTTGTAATCGTTGTTGCTG9770.10323439596783568No Hit
ATTATAATAGTCCCCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAA9740.10291740191675737No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9730.1028117372330646No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9720.10270607254937182No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9660.1020720844472152No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9640.10186075507982967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9540.10080410824290197No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9510.10048711419182368No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9490.10027578482443814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)