FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681516
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACAC50250.2988374776094905No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49720.29568555993520135No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATG46430.27611988229668943No Hit
GGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC42270.25138030206075945No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41230.24519540700177694No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37060.2203963566210491No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTA36600.21766072996034533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35250.20963226041262767No Hit
CCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGT33070.1966677688466836No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT28380.16877627093646447No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28290.1682410396332833No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27130.1613425028367259No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25840.15367085415779572No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG25600.1522435706826459No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25490.1515893990898689No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25260.15022158575951702No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC25060.14903218286355882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25020.1487943022843672No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGCGACTGAAGGTGATTCCAGAGGCGGCACA24370.14492874287250315No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23940.14237152664619307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23790.14147947447422446No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23510.13981431041988301No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT23380.1390411985375102No Hit
GGATAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC23250.1382680866551374No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23050.1370786837591792No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23030.1369597434695834No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC23020.1369002733247855No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTAC22600.13440252724327334No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT22500.13380782579529427No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22410.13327259449211307No Hit
GTTTTACTTCCAATGTTGTATCCGCCACAGGTAATCCTGGCCATCTGTCCTGGGGCCACTGACACCGAGGGTGG22290.13255895275453816No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21990.1307748484106009No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21930.13041802754181345No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGG21420.12738505015712012No Hit
GTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTA21390.1272066397227264No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21390.1272066397227264No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21310.12673087856434315No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA21220.12619564726116195No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21010.12494677422040586No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20430.12149750582212719No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCATCATAGA20430.12149750582212719No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGCTATTCGGCG20410.12137856553253136No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20360.12108121480854182No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20000.11894028959581711No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19830.11792929713425265No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19320.11489631974955933No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA19010.11305274526082415No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCG18740.11144705135128063No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC18400.10942506642815174No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18350.1091277157041622No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18270.10865195454577894No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18230.1084140739665873No Hit
CCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG18060.10740308150502284No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTAC17890.1063920890434584No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17740.10550003687148977No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17620.10478639513391487No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG17590.10460798469952115No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17250.10258599977639225No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTG17140.10193182818361526No Hit
TAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCC17110.10175341774922154No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17100.10169394760442363No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17060.10145606702523198No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16990.10103977601164664No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16970.10092083572205081No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16940.10074242528765709No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16930.10068295514285919No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16880.10038560441886964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)