FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511328
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36030.23839960617417266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31950.21140348091215144No Hit
GGTCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCA28360.18764953736052No Hit
GTAATACATAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTCCAGATACA28350.18758337038683862No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26340.17428380867687226No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGCATGCCATAACTAC25310.16746861038768554No Hit
ATGTATTACTGTGCGAAAGAGCAAACGGTGGTGACAAAGTACTACTTTGA23790.157411230388109No Hit
GGCTAACAGATTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23600.15615405788816195No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23590.15608789091448053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22310.14761851828325817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21990.14550117512545258No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCT21260.1406709860467086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20540.13590696394164603No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19600.1296872684155921No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19310.1277684261788308No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19040.12598191788943233No Hit
GTATTACTGTGCGAAAGAGCAAACGGTGGTGACAAAGTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC18870.12485707933684811No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18170.1202253911791484No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18120.11989455631074128No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17570.11625537275826293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGGGAAT17510.11585837091617437No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17490.11572603696881154No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17030.11268235617946601No Hit
GCATAGACCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC16870.11162368460056321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16750.11082968091638613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16730.11069734696902327No Hit
GAGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGGTCTATGCTGCATC16600.10983717631116476No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16290.10778600012704058No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16250.10752133223231489No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16230.10738899828495205No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA16190.10712433039022635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16110.10659499460077494No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15590.10315431196934086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG200.00710865152.20577645
GTGGTAT3150.038.6794781
GTATCAA30150.034.0129241
TATACCG450.00332587332.80067466
GACAGGT2850.032.063251
CGTATTC450.003958417631.65498757
GACCCGT553.392279E-431.147536
TATCAAC33600.030.0996652
CTATAAC450.005077836630.0840361
TCAACGC33700.029.8911744
ATCAACG33750.029.8508433
CAACGCA34400.029.381195
TCTGGAG15700.029.23053470
AACGCAG35000.028.974096
ACGCAGA36300.028.1226947
CGCAGAG36950.027.5364958
TGCACAG13800.027.01970970
AGAGTAC37800.026.734911
GCAGAGT38500.026.4278859
CGGGATT659.6823525E-426.03512617
Produced by FastQC (version 0.11.5)