FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268362
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC39660.3126867566199555No Hit
GTAATACACGGCTGTGTCCTCGCTTTTCAGGCCGTTCAATTGCAGATATA27760.21886496126500163No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26930.2123210881436057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25880.20404269443581566No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC25350.19986407665950257No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25280.1993121837456499No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25080.19773534684892796No Hit
GGGTATAACATTTTGACTGCCTGGGGCCACGGAACGCTGGTCACCGTCTC24890.19623735179704216No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23460.18496296798548048No Hit
GTATAACATTTTGACTGCCTGGGGCCACGGAACGCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT23100.18212466157138105No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22170.17479237000162412No Hit
GATCTATGGTGCATCCGGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA22020.17360974232908272No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21360.1684061805699004No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGAATCACTGTCAATACCGCCTGG21360.1684061805699004No Hit
GGATATTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC21230.16738123658703113No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21120.1665139762938341No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC20990.16548903231096485No Hit
GGGCTGGAGTGGGTTGGCCGTATTAAAAGCACAACTGATGGTGGGGCAAC20810.16406987910391513No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20770.16375451172457076No Hit
CCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCA20370.16060083793112692No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20160.15894515918956892No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19410.15303202082686174No Hit
GTATCAACGCAGAGTACGGGAAAATGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG19270.1519282349991564No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19180.15121865839563153No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18680.14727656615382675No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18300.14428057605005512No Hit
CTGTCAATACCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGACGGGG18150.14309794837751366No Hit
CTATTATTTGTCGGGCGAGTCAGGATATTAGCAGATGGTTAGCCTGGTAT18080.142546055463661No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17770.14010195827374206No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA17560.13844627953218402No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17440.13750017739415088No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAATTCATCCAGGCGGTAT17350.13679060079062602No Hit
GGGTAACATTTTCCCGTACACCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCAT17290.13631754972160945No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17190.13552913127324848No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA17020.13418881991103487No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16800.13245429932464076No Hit
ATGTTATACCCTGTGGAACAGTAATACACGGCTGTGTCCTCGCTTTTCAG16660.1313505134969354No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16650.13127167165209933No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16280.12835452339316378No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16200.127723788634475No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16050.12654116096193357No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15800.1245701148410312No Hit
GTGGGAGACAGAGTCACTATTATTTGTCGGGCGAGTCAGGATATTAGCAGATGGTTAGCCTGGTATCAGCAGAAA15760.1242547474616868No Hit
GTTATACCCTGTGGAACAGTAATACACGGCTGTGTCCTCGCTTTTCAGGC15710.12386053823750633No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15490.12212601765111222No Hit
GTGTATTACTGTTCCACAGGGTATAACATTTTGACTGCCTGGGGCCACGG15350.12102223182340688No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15060.1187358183231601No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14990.11818392540930743No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14980.11810508356447134No Hit
CTCCTGTGCAGCCTCTGGAATCACTGTCAATACCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGACGGGG14970.11802624171963524No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC14820.11684361404709381No Hit
GCTAATATCCTGACTCGCCCGACAAATAATAGTGACTCTGTCTCCCACAG14810.11676477220225771No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14690.11581867006422457No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14660.11558214452971628No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT14470.11408414947783045No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCATCTGCTA14320.11290152180528903No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTGGGAGACAGAGTCACTATTATTT14250.11234962889143636No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14230.11219194520176418No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14160.1116400522879115No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14090.11108815937405883No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13930.10982668985668129No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13890.10951132247733691No Hit
ACCTTGATGGGACCCCACTTTGCAAACCGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG13880.10943248063250081No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13750.10840753664963158No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13400.10564807208036822No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13350.10525386285618775No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13290.10478081178717116No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13280.10470196994233508No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13280.10470196994233508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13260.10454428625266288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13250.10446544440782679No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13230.1043077607181546No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13120.10344050042495755No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12920.10186366352823563No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12880.10154829614889124No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGCGTTCCGTGGCCCCAGGCAGTCAAAATGTTATACC12750.10052335216602201No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12730.10036566847634983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)