FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268362
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC42420.3344471057947179No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31000.24440971899189665No Hit
GGGTATAACATTTTGACTGCCTGGGGCCACGGAACGCTGGTCACCGTCTC25590.20175628093556885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25180.19852376529728893No Hit
GTATAACATTTTGACTGCCTGGGGCCACGGAACGCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT24500.19316251984843444No Hit
GTAATACACGGCTGTGTCCTCGCTTTTCAGGCCGTTCAATTGCAGATATA23990.18914158576179357No Hit
GGGCTGGAGTGGGTTGGCCGTATTAAAAGCACAACTGATGGTGGGGCAAC23940.18874737653761306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23110.18220350341621713No Hit
GTATCAACGCAGAGTACGGGAAAATGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTG21530.16974649193211402No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC21460.16919459901826134No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20850.1643852464832595No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20840.16430640463842341No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20770.16375451172457076No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGAATCACTGTCAATACCGCCTGG20610.1624930422071932No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20580.16225651667268493No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20530.16186230744850444No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC20240.15957589394825766No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20170.159024001034405No Hit
GATCTATGGTGCATCCGGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA19440.15326854636137002No Hit
GTGGGAGACAGAGTCACTATTATTTGTCGGGCGAGTCAGGATATTAGCAGATGGTTAGCCTGGTATCAGCAGAAA18770.1479861427573516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18760.1479073009125155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18740.14774961722284333No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18600.14664583139513798No Hit
CCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCA18340.1445959434293995No Hit
GGATATTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATC18250.14388636682587463No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA18000.14191532070497226No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17770.14010195827374206No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17590.1386828050666923No Hit
CTGTCAATACCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGACGGGG17360.13686944263546214No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16870.13300619223849341No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16800.13245429932464076No Hit
GGGTAACATTTTCCCGTACACCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCAT16760.13213893194529638No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAATTCATCCAGGCGGTAT16560.13056209504857447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.13048325320373838No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16470.12985251844504958No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16140.12725073756545843No Hit
GTGTATTACTGTTCCACAGGGTATAACATTTTGACTGCCTGGGGCCACGG16070.12669884465160577No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16070.12669884465160577No Hit
ATGTTATACCCTGTGGAACAGTAATACACGGCTGTGTCCTCGCTTTTCAG15950.1257527425135726No Hit
CTCCTGTGCAGCCTCTGGAATCACTGTCAATACCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGACGGGG15950.1257527425135726No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT15940.12567390066873652No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15750.12417590561685071No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15460.12188949211660394No Hit
CTATTATTTGTCGGGCGAGTCAGGATATTAGCAGATGGTTAGCCTGGTAT15390.12133759920275126No Hit
GTTATACCCTGTGGAACAGTAATACACGGCTGTGTCCTCGCTTTTCAGGC15230.12007612968537373No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14530.11455720054684704No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14420.11368994025364998No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14390.11345341471914171No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14360.1132168891846334No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14160.1116400522879115No Hit
GCTAATATCCTGACTCGCCCGACAAATAATAGTGACTCTGTCTCCCACAG14150.11156121044307539No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13860.10927479694282861No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13850.10919595509799253No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13810.10888058771864814No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC13730.10824985295995938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13660.10769796004610672No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13330.10509617916651555No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13110.10336165858012145No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13110.10336165858012145No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13060.10296744935594096No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13020.10265208197659657No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13010.10257324013176049No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCATCTGCTA12890.10162713799372736No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12850.10131177061438296No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12840.10123292876954686No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12710.10020798478667763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)