FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531462
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATTCTTTCCCGGACACGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT68470.4470891213755222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64010.4179666227434961No Hit
GAATTATACTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC56550.36925499947109364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55670.3635088562432499No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46750.30526385897919767No Hit
GAGTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCCTC42810.2795368086181701No Hit
CGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA41430.270525811283597No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40330.26334313224879236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39430.25746639485667944No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35660.23284939489193984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34440.22488315087152017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33900.2213571084362524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33450.21841873974019596No Hit
GGCCAAGCGTGTCCGGGAAAGAATTATACTGTTGACAGTAATAAGTTGCA33260.21717809517963882No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAACAGTATAATTCTTTC33170.21659042144042753No Hit
GTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT31690.20692645328450854No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG30930.2019638750422799No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAG30790.20104971589239565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30570.19961318008543472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29950.19556476099309028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29430.1921693127220917No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29070.18981861776524656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27490.17950167878798168No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAGCGTG27180.17747746924180946No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27030.17649801300979065No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26390.17231899975317702No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26320.1718619201782349No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26290.17166602893183117No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26260.17147013768542738No Hit
ACTTATTACTGTCAACAGTATAATTCTTTCCCGGACACGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24800.16193676369377757No Hit
GTAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGATACA24350.15899839499772114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24020.15684359128727973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23850.15573354089099173No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT22890.14946502100607134No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22580.1474408114598991No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22400.14626546398147652No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT22170.14476363109238102No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22140.14456773984597723No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA21400.13973575576801775No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA21300.1390827849466719No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21260.1388215966181335No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21260.1388215966181335No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20910.13653619874342296No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20770.13562203959353875No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20680.13503436585432743No Hit
GTATATTACTGTGCGAAAGATAAAAGGGGGAGTAGTGGCTGGTTTGGACCTCAAGATCACTGGGGCCAGGGAACC19850.12961470803715666No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19750.12896173721581078No Hit
GTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTC19370.12648044809469644No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19240.1256315860269468No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG19150.1250439122877355No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18510.1208648990311219No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18120.11831831282787296No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGACGATTTTG17980.11740415367798875No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17950.11720826243158497No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17950.11720826243158497No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17800.11622880619956617No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17790.11616350911743158No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17600.11492286455687441No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17450.1139434083248556No Hit
CACCATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAAC17390.11355162583204807No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17040.1112662279573375No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17020.11113563379306832No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGC16800.1096990979861074No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16790.1096338009039728No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16720.10917672132903068No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTG16650.10871964175408856No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAAATACTA16620.10852375050768481No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16440.10734840302926224No Hit
GCCCCTAACCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT16250.10610775846870506No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16240.10604246138657047No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16210.10584657014016671No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16110.10519359931882084No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15760.10290820144411028No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15670.10232052770489898No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15660.10225523062276438No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15650.1021899335406298No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15420.10068810065153427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA300.00242833668.4701470
TCGTATG1850.050.2877340
CGTATGC2000.046.6093541
CCTAACG606.7957444E-945.016232
CGAGACT2150.042.4253323
ACGAGAC2250.042.0329922
CACGAGA2300.041.115221
ATCTCGT1850.040.72815337
ATCTCCG2250.040.50666810
TAGGCAT2300.039.78064329
ATGCCGT2400.039.05763644
GACTAGG2400.038.0433226
CTAGGCA2550.037.1851228
CCGTCTT2600.036.2516247
CTAACGC754.865251E-836.0129853
GAGACTA2650.035.7000524
CTTATAC2850.035.5391271
ACTAGGC2600.035.13299627
TCTCCGA2750.034.36929711
GTATCAA36950.033.533741
Produced by FastQC (version 0.11.5)