FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531462
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATTCTTTCCCGGACACGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT64670.4222762301643789No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61250.3999446280743499No Hit
GAATTATACTGTTGACAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC55870.3648147978859417No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44980.2937062754413756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44590.29115968923812674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40170.2622983789346389No Hit
CGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA38760.2530914903536621No Hit
GAGTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCCTC36440.2379425672984377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36310.23709370523068807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34810.2272991429104999No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32730.21371734982650564No Hit
GGCCAAGCGTGTCCGGGAAAGAATTATACTGTTGACAGTAATAAGTTGCA32090.20953833656989204No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAACAGTATAATTCTTTC30850.20144149838520314No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30480.1990255063462234No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29890.19517297850028273No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27320.17839162839169367No Hit
GTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCT26760.1747349917921568No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26610.17375553556013795No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTATTTGGTTGGCCTGGTATCAGCAGAAG26230.17127424643902364No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26200.17107835519261985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26190.17101305811048528No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25550.16683404485387165No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAGCGTG25520.1666381536074679No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG25470.16631166819679497No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25130.16409156740421899No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24600.16063082205108584No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA24310.15873720666918278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23910.15612532338379925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23640.1543623021661654No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23580.15397051967335787No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23110.15090155681303224No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22480.1467878406385532No Hit
ACTTATTACTGTCAACAGTATAATTCTTTCCCGGACACGCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22250.1452860077494577No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21770.14215174780699752No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21270.1388868937002681No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21270.1388868937002681No Hit
GTAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGATACA21100.13777684330398013No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20510.13392431545803946No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20420.13333664171882814No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19920.13007178761209875No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19750.12896173721581078No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA19270.12582747727335056No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19210.12543569478054303No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19200.12537039769840844No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19130.12491331812346633No Hit
GGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT18930.12360737648077459No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18370.11995073988123767No Hit
GTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG18350.1198201457169685No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18080.11805712449933461No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA17770.1160329149531624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17670.11537994413181653No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17500.11426989373552854No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17430.11381281416058642No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17260.11270276376429843No Hit
GTATATTACTGTGCGAAAGATAAAAGGGGGAGTAGTGGCTGGTTTGGACCTCAAGATCACTGGGGCCAGGGAACC17140.11191919877868338No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16820.10982969215037658No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16800.1096990979861074No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16500.10774018552206976No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16480.10760959135780057No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16370.10689132345432012No Hit
CACCATCAGCAGCCTGCAGCCTGACGATTTTGCAACTTATTACTGTCAAC16170.10558538181162838No Hit
GTATTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTC15980.1043447372510712No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15850.10349587518332155No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGACGATTTTG15810.10323468685478321No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15790.10310409269051403No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15760.10290820144411028No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15650.1021899335406298No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAAATACTA15340.10016572399445758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)