FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175471
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA129300.5943540502263648No Hit
GTATATTACTGTGCAAGCCCGTATTACTATGATAGTAGTGGTTATTCCCC85220.3917312618738655No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73320.33703046374785045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72640.3339047038549353No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA55720.2561284429900468No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55470.2549792665588279No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54860.2521752760666541No Hit
CATTAATAGTGATGGGAGTAGCACAGGCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC48900.22477890994639782No Hit
GAGTATTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC45930.21112669394351843No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG45530.20928801165356836No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44980.206759823504887No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43460.19977283080307665No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT40910.18805123120464487No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG40700.1870859230024211No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40610.18667221948718232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40470.1860286806856998No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT39320.1807424691020933No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTA39000.1792715232701332No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38680.17780057743817315No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCTCCGCTCACTTTCGGCGG38190.1755481916329843No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAAC37960.17449094931626302No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCCACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG37180.17090551885086033No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA36050.16571124138175136No Hit
GAGTAGCACAGGCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCA36000.1654814060955076No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35870.16488383435127382No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35600.1636427238055575No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35050.16111453565687614No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34170.15706943461898595No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34150.15697750050448844No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT33710.15495494998554335No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC33270.15293239946659826No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32790.15072598071865817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32590.14980663957368312No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32430.1490711666577031No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32420.14902519960045432No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC32270.14833569374172306No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31540.14498009856256414No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC31120.14304948215811655No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG30450.13996968932245016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29410.13518911536857997No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA28860.13266092721989858No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28640.13164965196042602No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTGCCACGCTG28330.13022467318571473No Hit
CTATTAATGCGTGAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGAC27980.1286158261820084No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27960.12852389206751091No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA27750.1275585838652871No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27000.12411105457163069No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26470.12167480053744684No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25970.11937644767500923No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT25030.11505554429362654No Hit
GTGCTACTCCCATCACTATTAATGCGTGAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGTGCATC24570.11294105966018393No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC24550.11284912554568644No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC24550.11284912554568644No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24500.11261929025944267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24360.11197575145796014No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24260.11151608088547263No Hit
GTATTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC24110.11082657502674133No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23990.11027497033975632No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC23900.10986126682451755No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT23760.10921772802303502No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAAAGTGGGGAATAACCA23440.10774678219107495No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23380.10747097984758244No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23130.10632180341636362No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCA23080.10609196813011987No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC22920.10535649521413984No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22810.10485085758440355No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22740.1045290881836623No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA22650.10411538466842353No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22650.10411538466842353No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22550.103655714095936No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22540.10360974703868726No Hit
GTCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCAAGCCCGTATTACTATGATAGTAGTGGTTATTCCCCA22450.1031960435234485No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22390.10292024117995596No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC22360.10278234000820972No Hit
GGCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC22330.10264443883646346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG34900.050.56759370
TCGCACA305.402574E-447.47610558
GGTATCA19550.042.925481
GACAGGT3800.039.3399771
ACCCTGG36050.037.90638770
GTATCAA45200.035.1029361
AATGGTA6950.032.60571770
ACAGGTA3250.032.4073371
TATCAAC50400.030.926972
CGTTGTC32700.030.82550263
TGTCTCT33950.030.42207166
GCGTTGT32950.030.38238562
GTTGTCT33650.030.3761764
ATCAACG51600.030.2042683
GGCGTTG33350.030.0322361
TCAACGC52300.029.9250344
CGTGTTC33800.029.54232252
CAACGCA53550.029.2899045
AACGCAG54050.029.2073866
GTGTTCT34500.029.00228553
Produced by FastQC (version 0.11.5)