FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386765
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGAAGAGACA71840.5180401870540431No Hit
GTATATTACTGTGCGCGAGTAGGCACCCATTATTATGAGAGTAGTGGTTA55300.3987697987762887No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46180.333005231600163No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40760.2939214647038251No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39620.28570089380680935No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC31740.2288780002379639No Hit
GTAGTACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCGGCAGAGACAATTCCAAGAACACG31720.22873377969591097No Hit
GTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC31160.2246956045184296No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30900.2228207374717418No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29830.21510493847191123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29790.21481649738780542No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGAAGAGACAGCGTGTTCTTGGAATTGTCTCTG28960.20883134489260977No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT28440.2050816107992342No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT27890.20111554589277922No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25660.18503495545388007No Hit
CTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25280.18229476515487483No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25050.1806362289212664No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24910.17962668512689603No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24680.1779681488932876No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24520.17681438455686435No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23370.1685217033888222No Hit
CTCCTGTGCAGCCTCTGAATTCATTGTCAGCAGCACGTACATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG23220.16744004932342538No Hit
GCCCAAAAGTGTACCTCGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG23170.1670794979682931No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT22620.16311343306183818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22590.1628971022487588No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22410.16159911737028265No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22400.1615270070992562No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22020.15878681680025095No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21630.15597451623021924No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21450.15467653135174309No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21170.1526574437630024No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC20790.14991725346399715No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20760.14970092265091778No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTA20300.1463838501837009No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19910.14357154961366922No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACCTCGGAGGCCAGTTGCTACG19780.1426341160903253No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19680.1419130133800608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19040.13729795603436776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18960.13672107386615612No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18830.13578364034281223No Hit
GTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCGCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA18790.1354951992587064No Hit
ATGTACTACCATCCATATAAAGAACTGAGACCCACTCCAGCCCCTTCCCT18750.1352067581746006No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGAGGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAA18560.13383666302509797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18370.13246656787559535No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18310.13203390624943664No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACCTCGGAGG18210.1313128035391721No Hit
CATCTATGGTTCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18100.1305195905578811No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG17640.12720251809066424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17540.1264814153803997No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17480.126048753754241No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17410.12554398185705581No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17020.12273168128702412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17010.12265957101599767No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16870.12165002722162731No Hit
ATATTACTGTGCGCGAGTAGGCACCCATTATTATGAGAGTAGTGGTTACG16800.12114525532444215No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16760.12085681424033631No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT16600.11970304990391308No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16090.11602542608156394No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16090.11602542608156394No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16060.11580909526848455No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCGGCAGAGACAATTCCAAGAACACG15590.11241991253024125No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15470.1115545892779238No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15400.11104981738073863No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15350.11068926602560636No Hit
GTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTT15120.10903072979199793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15000.10816540653968047No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14820.10686742166120432No Hit
GCGTAGCAACTGGCCTCCGAGGTACACTTTTGGGCAGGGGACCAAGCTGG14780.1065789805770985No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14660.10571365732478105No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAATTTGTGTTGACACAGTC14650.1056415470537546No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14500.10455989298835779No Hit
GAGTTTGGCCTGAGCTGGGTTTTCCTTGCTGTTTTTTTAAAAGGTGTCCA14480.10441567244630488No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14400.10383879027809327No Hit
GAGTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATC14340.10340612865193453No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14320.10326190810988163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14220.1025408053996171No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14190.10232447458653772No Hit
CTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCAGCTCATGTACGTGCTGC14020.10109859997908802No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13900.10023327672677058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG16650.061.58061670
CGTATGC3150.049.22511741
TCGTATG3200.048.3542640
ATGCCGT3350.047.6135944
ATCTCGT3300.046.61773737
AATCTCG3400.045.15899736
CTTATAC3600.044.894721
GGCAATC3450.044.308633
AGGCAAT3800.042.86416232
GCAATCT3600.042.518134
TATACAC4000.042.0872763
TCTCCGA3850.041.10045611
ACGAGAC4050.040.75690522
GGTATCA8450.040.6443251
CACGAGA4000.040.4183621
GCCGTCT3550.040.2115846
AATGGTA5550.039.94418770
CCGTCTT3500.039.88345347
CTTGAAA4350.039.7287157
TAACCCG450.001300191339.71783469
Produced by FastQC (version 0.11.5)