FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386765
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGAAGAGACA64230.4631642708029118No Hit
GTATATTACTGTGCGCGAGTAGGCACCCATTATTATGAGAGTAGTGGTTA51530.37158422659931567No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48060.34656196255313626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42910.30942517297451266No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37820.2727210450220477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34290.24726611934970957No Hit
GTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC30800.22209963476147726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30740.22166697313531852No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAC30370.21899889310733975No Hit
GTAGTACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCGGCAGAGACAATTCCAAGAACACG30100.2170519157896255No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGAAGAGACAGCGTGTTCTTGGAATTGTCTCTG29370.21178786600469438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29260.2109946530234034No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT28190.20327885402357282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26980.19455351122937198No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26820.1933997468929487No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT26690.19246231336960481No Hit
CTCCTGTGCAGCCTCTGAATTCATTGTCAGCAGCACGTACATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG23970.17284831965040942No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23680.17075712179064226No Hit
GCCCAAAAGTGTACCTCGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG23670.1706850115196158No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23560.16989179853832478No Hit
CTTTTGGGCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23520.16960335745421898No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT22740.16397875631415562No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22640.16325765360389108No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTACCTACTTAGCCTGGTA21570.15554185460406053No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20930.1509267972583675No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20780.14984514319297068No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20770.14977303292194424No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20580.14840293777244162No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20470.14760972479115061No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20440.14739339397807127No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20040.14450898313701313No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19990.14414843178188083No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA19340.13946126416516136No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC19160.1381632792866852No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19090.13765850738950003No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACCTCGGAGGCCAGTTGCTACG19070.13751428684744713No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18840.1358557506138387No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18800.13556730952973287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18760.13527886844562706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18610.13419721438023025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18420.13282711923072762No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGCCCAAAAGTGTACCTCGGAGG18410.13275500895970116No Hit
ATATTACTGTGCGCGAGTAGGCACCCATTATTATGAGAGTAGTGGTTACG18210.1313128035391721No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17810.12842839269811396No Hit
GTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCGCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGA17740.12792362080092878No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17600.1269140770065584No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17480.126048753754241No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG17420.12561609212808225No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17410.12554398185705581No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17040.12287590182907702No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGAGGTACACTTTTGGGCAGGGGACCAAGCTGGAGATCAAA17040.12287590182907702No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16940.1221547991188125No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16930.12208268884778603No Hit
CATCTATGGTTCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16920.12201057857675957No Hit
ATGTACTACCATCCATATAAAGAACTGAGACCCACTCCAGCCCCTTCCCT16750.12078470396930986No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16540.11927038827775434No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAATTTGTGTTGACACAGTC16170.11660230824977555No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15460.11148247900689735No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15000.10816540653968047No Hit
GGGCTGGAGTGGGTCTCAGTTCTTTATATGGATGGTAGTACATACTACGC14920.10758852437146886No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14820.10686742166120432No Hit
CTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCAGCTCATGTACGTGCTGC14790.10665109084812495No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14770.10650687030607205No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14530.10477622380143715No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14220.1025408053996171No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14220.1025408053996171No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAAGTAGGCGTAAC14080.10153126160524675No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT14020.10109859997908802No Hit
GAGTGTTAGTACCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATC13970.10073804862395574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)