FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841571
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTCCAGGGAGA46220.5492109400157563No Hit
GTGTATTACTGTGCGACTTTCAGCGCTGGCAACAACTACTGGTACTTCGA27670.3287898466083075No Hit
GTCTCTGGTTACTCCATTAGCAGTGCTTACAACTGGGGCTGGATTCGGCA26850.3190461648512128No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20110.23895785382338505No Hit
CACCTGCACTGTCTCTGGTTACTCCATTAGCAGTGCTTACAACTGGGGCTGGATTCGGCAGCCCCCAGGGAAGGG19750.23468013988124592No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18860.22410468041317963No Hit
GTGTAGGAGCCCGCATATGAACAGCAGTAATAATCAGCCTCATCCTCAGC18770.22303525192764484No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18050.2144798240433665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18030.21424217326880324No Hit
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAAAGTGTAGGAGCCCGCATATGAACAGCAGTAATAATCAG17040.20247845992792052No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17000.20200315837879393No Hit
CTCCTACACTTTGTTCGGCGGAGGGACCAAACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT16970.20164668221694904No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16710.19855722214762628No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16080.19107122274888275No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC15370.18263462025188607No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTT15150.1800204617316899No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15080.1791886840207184No Hit
CTCCTGGACAGGCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15070.17906985863343675No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAAAGT14760.1753862716277058No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTCCAGGGAGAACTGGTTCTTGGACGTATCCG14760.1753862716277058No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14340.17039560536187678No Hit
GTATTACTGTGCGACTTTCAGCGCTGGCAACAACTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA13720.16302843135041487No Hit
GCTTACAACTGGGGCTGGATTCGGCAGCCCCCAGGGAAGGGCCTGGAATG13690.16267195518856994No Hit
GTGTCCGCCTCTCCTGGACAGGCAGTCACCATCTCCTGCACTGGAACCAG13520.160651923604782No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12900.1532847495933201No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12520.14876938487661764No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12460.1480564325529278No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12410.14746230561651957No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTTCT12100.14377871861078864No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12040.14306576628709877No Hit
GATTATTACTGCTGTTCATATGCGGGCTCCTACACTTTGTTCGGCGGAGGGACCAAACTGACCGTCCTAAGTCAG12010.14270929012525382No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11950.14199633780156398No Hit
CCTCAGGACTACCTGATCGCTTCTCTGCCTCCAAGTCTGGCTCCACGGCCTCCCTGACCATCTCTGGGCTCCAGG11850.14080808392874755No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11830.14057043315418427No Hit
GGTTATAACCGTGTCTCCTGGTACCAGCACCGCCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAATAAG11810.14033278237962096No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11650.13843157618311466No Hit
GTACCAGCACCGCCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA11640.13831275079583305No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11600.13783744924670646No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCGTGTCTCCTGGTACCAGCACCGCCCAGGCAAAGC11570.1374809730848615No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11470.13629271921204508No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGTTCATAT11460.13617389382476344No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11410.1355797668883552No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11410.1355797668883552No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11370.13510446533922865No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11360.134985639951947No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11150.1324903068190325No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11110.13201500526990592No Hit
GTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11030.13106440217165277No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10880.12928202136242814No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10870.12916319597514647No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG10810.1284502436514566No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10750.12773729132776676No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGG10590.12583608513126046No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10540.12524195819485223No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10310.12250897428737445No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10280.12215249812552952No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10150.12060776809086815No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10000.1188253872816435No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9860.11716183185970049No Hit
GGACTACCTGATCGCTTCTCTGCCTCCAAGTCTGGCTCCACGGCCTCCCT9820.11668653031057391No Hit
GTCCCTCACCTGCACTGTCTCTGGTTACTCCATTAGCAGTGCTTACAACT9630.11442884795222269No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG9470.11252764175571639No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9450.11228999098115311No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9440.11217116559387147No Hit
CCATTAGCAGTGCTTACAACTGGGGCTGGATTCGGCAGCCCCCAGGGAAG9430.11205234020658981No Hit
CTCCATCACTACCCTAGGGCTTCTTACTACAACCCGTCCCTCAAGAGTCG9400.1116958640447449No Hit
GGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCCTCTCCTGGACAGGCAGTCAC9350.11110173710833667No Hit
GTGCTGGTACCAGGAGACACGGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGC9270.11015113401008352No Hit
ATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCCTCTCCTGGACAGGCAGTCACCATCTCCTG9270.11015113401008352No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9130.10848757858814052No Hit
GTCCTGAGGGCCGCTTATTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGCGGTGCTGGTACCAGGAGA9110.10824992781357723No Hit
GTGCAAGAACATGAACCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCGCAGCTGCA9100.10813110242629558No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8950.10634872161707093No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT8890.10563576929338107No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8820.10480399158240955No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8810.10468516619512792No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8710.10349691232231148No Hit
CCCTAGGGCTTCTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCAGCGGATACGTCCAAGAACCAGTT8630.10254630922405833No Hit
ACCCTAGGGCTTCTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATT8630.10254630922405833No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8480.10076392841483368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAATCG100.009721472130.465770
TCGTATG1300.053.1173440
CGGTTGC200.006674316253.0426157
GGTATCA7000.049.24511
CGTATGC1500.048.40079541
ATCTCGT1450.047.4484437
ACGAGAC1700.046.17075722
GATCTCG1500.045.81194736
GTGGTAT1450.044.7224161
ATGCCGT1650.044.1895944
CTTATAC1650.043.4385221
GCCGTCT1700.042.98865546
CCCACGA1750.042.8989719
ATCTCCG1800.041.7147710
TATACAC1850.040.5873453
TGGTATC1600.040.5296942
GTATGCC1800.040.40209642
CGAGACA1950.040.25860623
TCCCGAC350.001366949539.31442337
TACACAT2100.039.0060165
Produced by FastQC (version 0.11.5)