FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841571
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTCCAGGGAGA45310.5383978297731267No Hit
GTGTATTACTGTGCGACTTTCAGCGCTGGCAACAACTACTGGTACTTCGA27740.3296216243192791No Hit
GTCTCTGGTTACTCCATTAGCAGTGCTTACAACTGGGGCTGGATTCGGCA26370.3133425462616939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18410.21875753798550568No Hit
GTGTAGGAGCCCGCATATGAACAGCAGTAATAATCAGCCTCATCCTCAGC18110.2151927763670564No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17970.21352922094511334No Hit
CACCTGCACTGTCTCTGGTTACTCCATTAGCAGTGCTTACAACTGGGGCTGGATTCGGCAGCCCCCAGGGAAGGG17530.20830090390472106No Hit
CTCCTACACTTTGTTCGGCGGAGGGACCAAACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT16910.20093372989325917No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16840.20010195218228763No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16290.19356655588179728No Hit
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAAAGTGTAGGAGCCCGCATATGAACAGCAGTAATAATCAG16030.19047709581247452No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15850.18833823884140494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15730.18691233419402523No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15600.18536760415936385No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15550.18477347722295562No Hit
CTCCTGGACAGGCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15240.18108989021722469No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15140.17990163634440826No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14200.16873204993993376No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC14030.16671201835614582No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTCCAGGGAGAACTGGTTCTTGGACGTATCCG14010.16647436758158254No Hit
GCTTACAACTGGGGCTGGATTCGGCAGCCCCCAGGGAAGGGCCTGGAATG13990.16623671680701926No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTT13930.1655237644833294No Hit
GTATTACTGTGCGACTTTCAGCGCTGGCAACAACTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA13810.16409785983594966No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13660.16231547902672502No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAAAGT13590.16148370131575351No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13090.15554243195167133No Hit
GTGTCCGCCTCTCCTGGACAGGCAGTCACCATCTCCTGCACTGGAACCAG13030.1548294796279815No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12990.1543541780788549No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12270.14579875019457658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTTCT12210.1450857978708867No Hit
GATTATTACTGCTGTTCATATGCGGGCTCCTACACTTTGTTCGGCGGAGGGACCAAACTGACCGTCCTAAGTCAG12170.14461049632176012No Hit
GGTTATAACCGTGTCTCCTGGTACCAGCACCGCCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAATAAG12130.14413519477263356No Hit
GTGCTCCCTTCATGCGAGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11970.14223398857612726No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11900.14140221086515575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11690.13890687773224125No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG11180.13284678298087743No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACCGTGTCTCCTGGTACCAGCACCGCCCAGGCAAAGC11100.13189617988262428No Hit
GTACCAGCACCGCCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA11050.13130205294621605No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11010.1308267513970895No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10990.1305891006225262No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10860.12904437058786483No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10790.12821259287689332No Hit
CCTCAGGACTACCTGATCGCTTCTCTGCCTCCAAGTCTGGCTCCACGGCCTCCCTGACCATCTCTGGGCTCCAGG10710.1272619897786402No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10640.1264302120676687No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGTTCATAT10640.1264302120676687No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGG10630.12631138668038705No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10170.12084541886543144No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10170.12084541886543144No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10130.12037011731630487No Hit
GGACTACCTGATCGCTTCTCTGCCTCCAAGTCTGGCTCCACGGCCTCCCT10110.12013246654174158No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10090.1198948157671783No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10080.11977599037989665No Hit
CTCCATCACTACCCTAGGGCTTCTTACTACAACCCGTCCCTCAAGAGTCG9890.11751830802154543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9850.11704300647241886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9820.11668653031057391No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9750.11585475259960241No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9730.11561710182503913No Hit
ATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCCTCTCCTGGACAGGCAGTCACCATCTCCTG9620.11431002256494104No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9580.11383472101581446No Hit
GTCCCTCACCTGCACTGTCTCTGGTTACTCCATTAGCAGTGCTTACAACT9490.11276529253027968No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9450.11228999098115311No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9410.11181468943202653No Hit
GTGCTGGTACCAGGAGACACGGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGC9340.11098291172105502No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG9320.11074526094649174No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9290.11038878478464682No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9260.11003230862280187No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9100.10813110242629558No Hit
GTGCAAGAACATGAACCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCGCAGCTGCA9060.107655800877169No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCAGTAGTTGTTGCCAGCGCTG9060.107655800877169No Hit
GGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCCTCTCCTGGACAGGCAGTCAC8960.10646754700435257No Hit
GTCCTGAGGGCCGCTTATTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGCGGTGCTGGTACCAGGAGA8940.10622989622978928No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8820.10480399158240955No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCTTGGGCTCAGTCTGCCCTGACTCAGC8780.10432869003328298No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC8780.10432869003328298No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8740.1038533884841564No Hit
CCCTAGGGCTTCTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCAGCGGATACGTCCAAGAACCAGTT8530.10135805535124191No Hit
GGCCTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTTACTCCATTAGCAGTGCTTACAA8500.10100157918939696No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8490.10088275380211534No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8420.10005097609114381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC150.002472057568.163363
CCTAACG200.007714682751.1346782
GTGGTAT1800.047.372261
GGTATCA7550.044.272671
AGGTATC504.1775456E-640.8980143
GTATCAA18400.036.8885771
TGGTATC2400.035.510192
CGCTGAA19600.034.0811170
GCAGTTT800.002895213733.73685570
TATCAAC21550.031.321472
ATCAACG21950.030.8986533
TCAACGC22350.030.3438534
TCCGCTG12700.030.2131368
AAAGTCA2251.7043931E-929.98831470
TATCCGC12100.029.95379466
CAACGCA22700.029.8759965
AACGCAG22700.029.7258666
AATGGTA2501.6189006E-1029.6884370
CCGAGTT604.5764717E-429.59372959
ATCCGCT12200.029.54541467
Produced by FastQC (version 0.11.5)