FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916693
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30000.3272633258899108No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATAAGCGCTGTTGTGCCAAATCATA27460.29955503096456504No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26390.28788263900782485No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCC25560.278828353658204No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25330.2763193348263813No Hit
CACCTGCACCTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC23860.2602834318577757No Hit
GACTATTACTGTATGATTTGGCACAACAGCGCTTATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT21770.2374840868207786No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20490.22352085158280904No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATAAGCGCTGTTGTGCCAAATCATACAGTAATAGT20490.22352085158280904No Hit
GTGCTGACTCAGCCGGCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG18580.20268508650115144No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18410.20083059432110859No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18010.19646708330924315No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17870.19493985445509018No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17460.19046725566792808No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16870.1840310769254265No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16430.17923121481237447No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15760.1719223338674998No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGCCGG15540.1695224028109738No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14430.15741365975304708No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC14210.15501372869652108No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG14150.15435920204474124No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGGCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC14030.1530501487411816No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13990.15261379763999505No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13910.151741095437622No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT13630.14868663772931615No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13580.14814119885283294No Hit
CCCAATAAGCGCTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCC13190.14388677561626412No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13140.14334133673978094No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT13120.14312316118918766No Hit
CTCCAGAGCCCTGGTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG13110.14301407341389102No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13060.14246863453740785No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12980.14159593233503473No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12920.14094140568325492No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12830.13995961570558518No Hit
GATAAGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA12780.139414176829102No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12700.1385414746267289No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12490.13625063134549953No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGGTACA12350.13472340249134662No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC12220.13330526141249036No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12190.1329779980866004No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCACCAGGGCTC11920.13003262815359123No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGG11880.12959627705240467No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11850.12926901372651475No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11780.1285053992994383No Hit
ATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT11280.12305101053460646No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11260.12283283498401318No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11120.12130560612986026No Hit
CTCGCAGGCTGTGCTGACTCAGCCGGCTTCCCTCTCTGCATCTCCTGGAG10990.11988746505100399No Hit
CTATTACTGTATGATTTGGCACAACAGCGCTTATTGGGTGTTCGGCGGAG10890.11879658729803762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10670.1163966562415116No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG10650.11617848069091832No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10550.11508760293795195No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA10520.11476033961206206No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC10490.11443307628617214No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGGCTTCCCT10440.11388763740968896No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC10430.11377854963439234No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10400.1134512863085024No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10020.1093059508472302No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9710.10592422981303445No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9680.10559696648714456No Hit
GTATATCCTGTAGGTACCAACATTGATGCCACTGCGCAAGGTGCAGGTGA9680.10559696648714456No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTAGG9670.10548787871184791No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9540.10406973763299164No Hit
GTACAAATCAGACTCAGATAAGCACCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC9390.10243342100354207No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9250.10090619214938917No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9240.10079710437409252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)