FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061970
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAGA39520.3721385726527115No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30290.2852246296976374No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27920.26290761509270505No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25830.24322720980818668No Hit
GTGTTGCTGCTTGCGTATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC23690.2230759814307372No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22910.21573114118101266No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21200.19962899140277035No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAAAAGCACAGTGTTGCTGCTTGCGTATGAGCTG21100.19868734521690817No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19580.1843743231918039No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18550.17467536747742404No Hit
CAGCAACACTGTGCTTTTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18280.1721329227755963No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18130.17072045349680312No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17750.16714219799052704No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17060.16064483930807838No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16950.15960902850363004No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC16600.15631326685311261No Hit
GTTTACTACTGTGCGAAAGATTACCATAACTGGAACTACGGGTGGTTCGA16200.1525466821096641No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAAAAGCACAGTGTTGCTGCTTGCGTATGAGCTGCAGTAATAAT16020.1508517189751123No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15970.15038089588218123No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15870.1494392496963191No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15500.14595515880862925No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTC14930.14058777554921514No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14820.1395519647447668No Hit
GATTATTACTGCAGCTCATACGCAAGCAGCAACACTGTGCTTTTCGGCGGAGGGACCACGCTGACCGTCCTAGGT14590.1373861785172839No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT13960.13145380754635252No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13850.13041799674190419No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13830.13022966750473175No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13330.12552143657542117No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13170.12401480267804174No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13160.12392063805945552No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12870.12118986412045538No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12750.12005988869742082No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12660.11921240713014493No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12410.11685829166548961No Hit
GTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTCGGTGGTTATAACTATGTCTCCTGGTACCAACAACA12340.11619913933538613No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12190.11478667005659293No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT12180.11469250543800673No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC12040.11337420077779975No Hit
CTCCTGCACTGGAACTAGCAGTGACGTCGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC11990.11290337768486869No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCACCCGTAGTTCCAGTTATGGTAATCTTT11940.11243255459193763No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11840.11149090840607549No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11830.11139674378748927No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11810.11120841455031687No Hit
GGTTATGACACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACT11740.11054926222021338No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCACCCGTAGTTCCAGTTATGGTAA11710.11026676836445473No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11690.11007843912728232No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11670.10989010989010989No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11320.10659434823959246No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11250.10593519590948897No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC11220.10565270205373033No Hit
GGTCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11210.10555853743514412No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCACAGCTGC11070.10424023277493714No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC11000.10358108044483366No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAC10980.10339275120766123No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10970.10329858658907504No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10900.10263943425897154No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT10740.10113280036159213No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10730.10103863574300591No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10650.10028531879431622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT200.007099764452.2212348
CTAACGC306.5846846E-445.6057323
AGGGGGG3050.044.46287570
GGTATCA7400.044.3794141
CGTATGC2950.043.38862641
TCGTATG2950.043.3205440
CTTATAC3050.041.4996031
TAGGCAT3050.040.43321629
TCTGGAG14450.040.22099370
TATACAC3350.039.819933
GACTAGG3200.039.5748126
TACACAT3200.039.548725
GTATCAA19950.039.096151
CGGGTAT350.001406346339.08878717
ATGCCGT3350.038.31006644
GTATGCC3350.038.25526442
ATCTCGT2800.038.12035837
CGAGACT3450.037.67607523
ACGAGAC3450.037.6707522
ACTAGGC3400.037.26092527
Produced by FastQC (version 0.11.5)