FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061970
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATAGA37180.35010405190353777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35560.33484938369257133No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32900.3098015951486388No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25590.24096725896211754No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24440.23013832782470314No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24110.22703089541135815No Hit
GTGTTGCTGCTTGCGTATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC23700.22317014604932342No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19750.18597512170776953No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19170.1805135738297692No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAAAAGCACAGTGTTGCTGCTTGCGTATGAGCTG19090.1797602568810795No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18690.175993672137631No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17720.1668597041347684No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17690.16657721027900976No Hit
CAGCAACACTGTGCTTTTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA16590.1562191022345264No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16570.15603077299735396No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTGTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15820.14896842660338805No Hit
GATTATTACTGCAGCTCATACGCAAGCAGCAACACTGTGCTTTTCGGCGGAGGGACCACGCTGACCGTCCTAGGT15470.1456726649528706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC15070.1419060802094221No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15030.14152942173507727No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14970.14096443402356No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTC14660.1380453308473874No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT14630.13776283699162875No Hit
GTTTACTACTGTGCGAAAGATTACCATAACTGGAACTACGGGTGGTTCGA14570.13719784928011147No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14230.13399625224818026No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14080.13258378296938708No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAAAAGCACAGTGTTGCTGCTTGCGTATGAGCTGCAGTAATAAT13960.13145380754635252No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13830.13022966750473175No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13740.12938218593745587No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13470.1268397412356281No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13340.1256156011940074No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13100.12335565034793826No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC13030.12269649801783479No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC12980.1222256749249037No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12760.12015405331600704No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCACCCGTAGTTCCAGTTATGGTAA12690.11949490098590355No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12420.11695245628407583No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT12260.11544582238669641No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11940.11243255459193763No Hit
CTCCTGCACTGGAACTAGCAGTGACGTCGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC11930.11233838997335142No Hit
GTCGATCACCATCTCCTGCACTGGAACTAGCAGTGACGTCGGTGGTTATAACTATGTCTCCTGGTACCAACAACA11890.11196173149900655No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11880.11186756688042036No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11510.1083834759927305No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCACCCGTAGTTCCAGTTATGGTAATCTTT11470.10800681751838563No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11470.10800681751838563No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAC11430.10763015904404079No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11190.1053702081979717No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA11050.10405190353776472No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11020.10376940968200608No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10850.10216861116604047No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT10640.10019115417573002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)