FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261990
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAACGTAGCAACTGGCCCCGAGGAATTTTCGGCCCTGGGACCAGAGTGGCTATCAAACGAA45110.3574513268726377No Hit
GTGTATTACTGTCAGCAACGTAGCAACTGGCCCCGAGGAATTTTCGGCCC44960.3562627279138504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39060.30951116886821606No Hit
CAGTAATACACTGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34290.2717137219787795No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32160.25483561676399974No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTGCTGTGGCTCCCAGATACCA28730.22765632057306318No Hit
GTAATACACTGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA27880.22092092647326844No Hit
GTCTCCAGCCGTCCTGTACTTGTCTCCAGGGGAGACAGCCACCCTCTCCT26760.2120460542476565No Hit
CAATAATACAAGGCCGTGTCCTCAGGTCTCAGATTGTTCATTTGCAGATA26690.21149137473355575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26000.2060238195231341No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24840.1968319875751789No Hit
CTCCAGGCCCATCCCTGGAGCTTGTCGGACCCAGTGCATGGCATGATTAT24550.19453402958819008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24010.19025507333655575No Hit
GTCCTGTACTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAACAGGTTCTTGGC20900.1656114549243655No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20580.16307577714561922No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20490.16236261777034683No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20060.15895530075515654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19850.1572912622128543No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19240.15245762644711922No Hit
GTATTATTGTGCGAAAGATATCGGAAGTTCGGGAGATTATTATACTGGCG19060.15103130769657447No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATGCCAGCGGCCCTATT19030.150793587904817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18970.15031814832130208No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAACAGGTTCTTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACT18450.14619767193083938No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT18200.14421667366619387No Hit
GTATAATAATCTCCCGAACTTCCGATATCTTTCGCACAATAATACAAGGC18140.14374123408267894No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17980.14247339519330582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17780.1408885965815894No Hit
CAATAGGGCCGCTGGCATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17690.140175437206317No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17540.1389868382475297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17430.13811519901108568No Hit
GCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGG17290.13700583998288415No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17200.1362926806076118No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16910.13399472262062298No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16650.13193448442539163No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16560.13122132505011924No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCGCTGGCATCCCA16510.13082512539719016No Hit
GAGTTTAGGTTATGCGGAGTCTGTGAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAATTCCCTGTAT16460.13042892574426104No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16250.1287648872019588No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCCATTCTAAAAGGTGGCCAGTGTGAGGTACAACTGGTGGAGT16150.1279724878961006No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16020.12694236879848494No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15720.12456517088091032No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15670.12416897122798119No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACTCTGGTCCCAGGGCCGAAAATTCCTCGGGGCCAGTTGCTACGTTG15560.12329733199153718No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15350.12163329344923494No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15090.1195730552540036No Hit
GAGTGTGAACAGGTTCTTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC14960.11854293615638793No Hit
GCCCTATTGGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCCAAGAAC14960.11854293615638793No Hit
TTATTATACTGGCGGGGCGAGTGACCACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCGACCAG14770.11703737747525732No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACTCTGGTCC14390.11402626011299613No Hit
GCCTAGAGCCTGAAGATTATGCAGTGTATTACTGTCAGCAACGTAGCAAC14240.11283766115420882No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14100.11172830212600733No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14090.11164906219542153No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGGCTGGGATGCCA13940.11046046323663422No Hit
ATACAAGGCCGTGTCCTCAGGTCTCAGATTGTTCATTTGCAGATACAGGG13660.10824174518023122No Hit
GGCCTGGAGTGGGTCGCAGGTATTAATTGGAGTGGTCGGAGTTTAGGTTA13600.1077663055967163No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13590.10768706566613047No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13570.10752858580495885No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13510.1070531462214439No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13480.10681542642968646No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC13420.10633998684617153No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13080.10364582920625362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13060.10348734934508197No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13020.1031703896227387No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12930.10245723024746631No Hit
GTCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCCAAGAACCTGTTC12840.1017440708721939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2650.055.94972240
CGTATGC2850.052.1307541
ACGAGAC2850.051.0810722
ATCTCGT2700.048.271137
ATGCCGT3200.046.65352644
CTTATAC3300.046.1527181
TATACAC3200.045.4778563
GCCGTCT3000.045.31138646
GGTATCA10450.045.0191421
CGAGACT3250.043.7628523
GATCTCG3150.042.40445336
CACGAGA3450.042.19071621
TCTCCGA3550.041.9458111
ATCTCCG3600.041.3632310
CCGTCTT3300.041.2679547
TTATACA3700.041.1616062
GACTAGG3550.040.11388426
CCCACGA3600.039.48777819
ACTAGGC3700.039.43689327
GTATCAA28800.039.016141
Produced by FastQC (version 0.11.5)