FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261990
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTCAGCAACGTAGCAACTGGCCCCGAGGAATTTTCGGCCC45560.36101712374899964No Hit
GTATTACTGTCAGCAACGTAGCAACTGGCCCCGAGGAATTTTCGGCCCTGGGACCAGAGTGGCTATCAAACGAA44080.34928961402229813No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37650.2983383386556153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34690.2748833192022124No Hit
CAGTAATACACTGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33160.2627596098225818No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTGCTGTGGCTCCCAGATACCA27870.2208416865426826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26530.21022353584418263No Hit
GTAATACACTGCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA26210.20768785806543635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26130.20705393862074975No Hit
GTCTCCAGCCGTCCTGTACTTGTCTCCAGGGGAGACAGCCACCCTCTCCT25620.20301270216087292No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25370.20103170389622738No Hit
CAATAATACAAGGCCGTGTCCTCAGGTCTCAGATTGTTCATTTGCAGATA24570.19469250944936173No Hit
CTCCAGGCCCATCCCTGGAGCTTGTCGGACCCAGTGCATGGCATGATTAT22410.17757668444282443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22370.17725972472048115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22300.1767050452063804No Hit
GTCCTGTACTTGTCTCCAGGGGAGACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTGAACAGGTTCTTGGC21090.16711701360549608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20790.16473981568792145No Hit
GTATTATTGTGCGAAAGATATCGGAAGTTCGGGAGATTATTATACTGGCG19840.15721202228226847No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19540.15483482436469384No Hit
GTCCCAGACCCACTGCCACCGAACCTGGCTGGGATGCCAGCGGCCCTATT19160.15182370700243267No Hit
CTCCTGCAGGGCCAGTCAGAGTGTGAACAGGTTCTTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACT18740.1484956299178282No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT18210.14429591359677968No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17710.14033391706748866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17700.14025467713690282No Hit
GCATAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGG17580.139303797969873No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17570.13922455803928716No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17290.13700583998288415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16900.13391548269003717No Hit
GTATAATAATCTCCCGAACTTCCGATATCTTTCGCACAATAATACAAGGC16800.13312308338417894No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16680.13217220421714912No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16680.13217220421714912No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16660.13201372435597747No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCCATTCTAAAAGGTGGCCAGTGTGAGGTACAACTGGTGGAGT16470.13050816567484688No Hit
CAATAGGGCCGCTGGCATCCCAGCCAGGTTCGGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG16120.12773476810434314No Hit
GAGTTTAGGTTATGCGGAGTCTGTGAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAATTCCCTGTAT16010.1268631288678991No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15980.12662540907614164No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACTCTGGTCCCAGGGCCGAAAATTCCTCGGGGCCAGTTGCTACGTTG15640.12393125143622374No Hit
TTATTATACTGGCGGGGCGAGTGACCACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCGACCAG15480.12266341254685062No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAATAGGGCCGCTGGCATCCCA15440.12234645282450732No Hit
GCCCTATTGGATGCATCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCCAAGAAC15380.12187101324099239No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15330.12147481358806331No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14900.118067496572873No Hit
GAGTGTGAACAGGTTCTTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC14800.1172750972670148No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.114977139280026No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14510.114977139280026No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGGCTGGGATGCCA14450.11450169969651107No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14410.11418473997416778No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14370.11386778025182451No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14000.11093590282014913No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13910.11022274344487674No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13890.1100642635837051No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13890.1100642635837051No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13620.10792478545788795No Hit
GTGTGAGGTACAACTGGTGGAGTCTGGGGGAGGCTTGGCACAGCCTGGCAGGTCCCTGAGACTCTCCTGTGCAGG13450.10657770663792898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13130.1040420288591827No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13080.10364582920625362No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13060.10348734934508197No Hit
GTCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCCAAGAACCTGTTC12900.10221951045570885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACTCTGGTCC12870.10198179066395138No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC12810.10150635108043646No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTATGCAGTGTATTACTGTCAGCAACGTAGCAACTGG12650.10023851219106332No Hit
ATACAAGGCCGTGTCCTCAGGTCTCAGATTGTTCATTTGCAGATACAGGG12620.10000079239930586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.045.7372671
GTATCAA28750.038.7810971
TATCAAC32100.033.9889872
TCAACGC33450.032.6172334
ATCAACG33550.032.419333
CAACGCA34250.031.8591585
AACGCAG34600.031.5393836
ATAGACG450.00426183331.18051549
TGCACAG10100.031.0044370
ACGCAGA36100.030.2348757
TGTTAGA12650.030.13594270
CGCAGAG36550.029.9622048
AGAGTAC37550.029.17237311
CAGAGTA37900.028.98872810
GCAGAGT38850.028.1917329
TTGTCGC606.109117E-428.1699839
TAGACGG500.00703703428.1400450
AAACGAA21000.027.87830770
CGAACTA500.00836356827.16165529
ATCGTGC500.00852400627.05605716
Produced by FastQC (version 0.11.5)