FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139097
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62980.5528940906700659No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTACTACCTGCATATGAG58460.5132135366873936No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTA50790.44587949928759363No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48450.42533691160629866No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46040.4041798020712898No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCACCTG42450.3726636098593886No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41110.3608999058025787No Hit
CTCCTGGACAGTCGATCACCATCACCTGCACTGGAGCCAACAGCGATGTT40530.3558081533003774No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC39540.34711705851213726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36930.3242041722522314No Hit
GTATTACTACCTGCATATGAGCAGCAATGATAATCAGCCTCGTCCTCAGC35990.31595202164521546No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35530.31191373517795234No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCCAA33510.2941803902564926No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC32300.2835579410708658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32020.28109985365601No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31230.27416453559266685No Hit
GTCGATCACCATCACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA30350.26643911800312003No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30310.2660879626581406No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30270.2657368073131612No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28760.25248069304018883No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28310.2485301954091706No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27940.24528200846811116No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27800.24405296476068325No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27200.23878563458599225No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCA26840.23562523648117764No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26540.23299157139383214No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26280.23070906165146604No Hit
TAGTAATACTTTCGTGCCATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT26100.22912886259905874No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25880.227197508201672No Hit
CACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC25800.22649519751171324No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25500.22386153242436774No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25320.2222813333719604No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC24820.2178918915597179No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTAC24370.21394139392869965No Hit
CCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTACTACCTGCATATGAGCAGCAATGATAATCAGCCTCGTCCT23650.20762059771907043No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22850.20059749081948247No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22810.20024633547450305No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC20880.18330309007924697No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20390.17900143710324934No Hit
CCCCTGAGGGCCGCTTACTGGTCTCATAAATGATGAGTTGGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA20120.17663113852463838No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20110.17654334968839352No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19900.17469978412725168No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19900.17469978412725168No Hit
GATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCGTGCCATTCGG19740.17329516274733409No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG19450.17074928649623344No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19060.16732552188268426No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA18850.16548195632154242No Hit
CCATCACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTC18740.16451627912284905No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCTCCCTGACGATCTCTGGGCTCCAGG18690.16407733494162483No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18610.16337502425166603No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18060.15854663825819926No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC17950.15758096105950592No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17830.1565274950245677No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17660.15503508480840525No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17460.15327930808350826No Hit
GGTTATAACTCCCAACATCGCTGTTGGCTCCAGTGCAGGTGATGGTGATC17410.152840363902284No Hit
GCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC17120.15029448765118336No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17080.14994333230620394No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16970.1489776551075106No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC16800.14748524489134815No Hit
TCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCT16670.1463439900201651No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16640.14608062351143056No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16410.144061480277799No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT16340.14344695842408503No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16040.14081329333673953No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC16000.14046213799176016No Hit
ATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCCAACTCATCATTTATGAGA15610.13703837337821098No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15540.13642385152449704No Hit
ATGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGG15540.13642385152449704No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15480.13589711850702793No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15350.13475586363584488No Hit
GCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTAC15330.13458028596335517No Hit
GTTGGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA15290.13422913061837577No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCACCTGCA15190.1333512422559273No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15130.13282450923845818No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15090.13247335389347878No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT14750.12948853346115388No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14610.12825948975372597No Hit
GCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGT14300.12553803583013562No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT14220.12483572514017682No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14050.12334331492401439No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC14020.12307994841527982No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13960.12255321539781072No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13720.12044628332793432No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13670.12000733914671008No Hit
CAACAACACCCAGGCAAAGCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCG13580.11921723962050643No Hit
GGCAAAGCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGG13540.11886608427552701No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13510.11860271776679247No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13440.11798819591307852No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTA13350.11719809638687487No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC13310.11684694104189546No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC13210.11596905267944697No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13130.11526674198948816No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12870.11298423224712206No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCATTGCTGCTCATAT12740.11184297737593901No Hit
CATATGCAGGTAGTAATACTTTCGTGCCATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTG12610.11070172250475596No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12430.10912152345234866No Hit
GGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCG12430.10912152345234866No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12040.10569775883879949No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11870.10420534862263706No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC11760.1032396714239437No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11750.10315188258769885No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCC11660.1023617830614952No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG11660.1023617830614952No Hit
GGCTGAGGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCGTGCCATTCGGCGGAGGGAC11650.10227399422525037No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11640.10218620538900551No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11450.10051821750035335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)