FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139097
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60050.5271719616503248No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTACTACCTGCATATGAG55770.4895983397375289No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50010.43903197006049527No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTA44710.39250388685072474No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCACCTG43960.385919724132361No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43330.38038902744893544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41610.3652893476148212No Hit
CTCCTGGACAGTCGATCACCATCACCTGCACTGGAGCCAACAGCGATGTT39550.3472048473483821No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37710.33105170147932966No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36170.31753222069762277No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35860.3148107667740324No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC35120.30831439289191354No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34270.3008523418111012No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCCAA33850.2971652106888175No Hit
GTATTACTACCTGCATATGAGCAGCAATGATAATCAGCCTCGTCCTCAGC33600.2949704897826963No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33180.2912833586604126No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC32600.2861916061582113No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31040.27249654770401466No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29690.2606450548109599No Hit
GTCGATCACCATCACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA29280.2570457125249211No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28300.24844240657292574No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28090.24659884101178392No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26390.23167473885015938No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26140.22948001794403813No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25890.22728529703791686No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25670.22535394264053019No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCA25660.22526615380428533No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25270.22184238919073615No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25050.21991103479334947No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24940.21894535759465614No Hit
CACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC23950.21025426280641596No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23900.20981531862519173No Hit
TAGTAATACTTTCGTGCCATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT23290.20446019961425588No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23060.2024410563806243No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTAC21930.19252091788495623No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21880.19208197370373198No Hit
CCTTGGTCCCTCCGCCGAATGGCACGAAAGTATTACTACCTGCATATGAGCAGCAATGATAATCAGCCTCGTCCT21390.18778032072773435No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20650.18128394684561544No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG20510.18005490313818753No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20450.17952817012071842No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20360.17873807059451477No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20330.17847470408578023No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20150.17689450503337292No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20150.17689450503337292No Hit
CCCCTGAGGGCCGCTTACTGGTCTCATAAATGATGAGTTGGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA20010.17566546132594502No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19440.1706614976599886No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19230.1688179320988467No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC19190.16846677675386731No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA19030.16706215537394972No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18710.1642529126141145No Hit
GATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCGTGCCATTCGG18690.16407733494162483No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18460.16205819170799327No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC18240.16012683731060656No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18140.15924894894815805No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC18020.15819548291321986No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17910.1572298057145265No Hit
GGTTATAACTCCCAACATCGCTGTTGGCTCCAGTGCAGGTGATGGTGATC17600.15450835179093614No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCTCCCTGACGATCTCTGGGCTCCAGG17410.152840363902284No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT17190.1509090095048973No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16910.1484509220900415No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16880.14818755558130695No Hit
TCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCCT16820.14766082256383786No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16600.14572946816645116No Hit
CCATCACCTGCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTC16230.1424812812253917No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16170.14195454820792258No Hit
GTTGGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGGTTATAA16130.14160339286294318No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15910.13967203846555648No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC15460.13572154083453825No Hit
GCCTGGAGCCCAGAGATCGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC15360.13484365247208974No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCACCTGCA15270.13405355294588608No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14920.13098094367731633No Hit
ATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCCAACTCATCATTTATGAGA14770.12966411113364357No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14640.12852285626246054No Hit
GCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGT14250.12509909164891136No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14210.12474793630393197No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13820.12132417169038283No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC13820.12132417169038283No Hit
ATGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATCGTCAGGGAGG13800.12114859401789313No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13680.12009512798295491No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC13510.11860271776679247No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTC13480.11833935125805792No Hit
GCACTGGAGCCAACAGCGATGTTGGGAGTTATAACCTTGTCTCCTGGTAC13470.11825156242181306No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13250.11632020802442636No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13170.11561789733446756No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC13100.11500337548075361No Hit
GGCAAAGCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGG13100.11500337548075361No Hit
GGCTGAGGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCGTGCCATTCGGCGGAGGGAC13030.11438885362703965No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13010.11421327595454996No Hit
GGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTAGTAATACTTTCG12920.11342317642834632No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12870.11298423224712206No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12820.11254528806589781No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12660.1111406666859802No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATGG12530.10999941181479717No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12320.10815584625365532No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12310.10806805741741046No Hit
CAACAACACCCAGGCAAAGCCCCCCAACTCATCATTTATGAGACCAGTAAGCGGCCCTCAGGGGTTTCTAATCG12300.1079802685811656No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12280.10780469090867591No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCATTGCTGCTCATAT12150.10666343603749286No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATCATTGCTGCTCATATGCAGGTA12050.10578554767504435No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT11880.10429313745888191No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11860.10411755978639221No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11780.1034152490964334No Hit
GAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCC11710.10280072724271946No Hit
CCCTGACGATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCATTGC11690.10262514957022975No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11640.10218620538900551No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11490.10086937284533276No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11450.10051821750035335No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG11440.10043042866410849No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC11400.10007927331912911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.039.8055341
GTATCAA29050.035.5506671
GTGGTAT4300.035.4354631
TTGCAAT1001.7979245E-434.70043670
GACCGAA450.00384080631.84990958
TGGTATC4850.031.408732
TTGCCGA802.1289343E-631.21152356
CCTTATA553.6345609E-430.782321
TATCAAC33650.030.4815562
TAGCTCG450.0050808430.07977110
ATCAACG34850.029.5213343
TCAACGC36000.028.5757854
CAACGCA36800.027.9533425
TTTGCCG905.4425454E-627.66803755
AACGCAG37250.027.433976
AATGATA30650.026.2659170
AGAGTAC38950.026.06398811
CGCAGAG39150.026.016138
ACGCAGA39300.026.0029357
GCAGAGT39200.025.8977629
Produced by FastQC (version 0.11.5)