FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239189
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65650.5297819783745659No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59120.47708622332832196No Hit
GACTTACAGTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT54550.4402072645899859No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50020.40365109761303564No Hit
CTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC48240.3892868642313642No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47430.38275033106330025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43740.3529727910754534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43670.3524079054930281No Hit
GTCTCCATCCTCCGTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG42220.34070670414279014No Hit
ATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGT41340.33360528539230094No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40700.32844061721012696No Hit
GTCCCTAACCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT38200.3082661321235098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38090.30737845477969866No Hit
GATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA37860.3055224021517299No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37330.301245411313367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37060.2990665669240124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36940.2980981916398548No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36780.2968070245943113No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33770.27251694455002423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33590.2710643816237878No Hit
GTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGA33420.26969251663789784No Hit
GCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT33100.26711018254681085No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32480.26210691024532984No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC32160.2595245761542428No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31980.2580720132280064No Hit
CCTTCGGCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA31210.2518582718213283No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30420.24548313453395731No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGTACT30220.24386917572702793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29640.23918869518693275No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28610.23087680733124646No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTG27970.22571213914907248No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27590.2226456174159067No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA27420.22127375243001673No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC27420.22127375243001673No Hit
GGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTA27190.21941769980204795No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26610.21473721926195277No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA25390.2048920705396836No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24880.20077647558201372No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC24160.194966223877068No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24050.19407854653325682No Hit
GATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAAT23790.19198040008424866No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23710.19133481656147688No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23680.1910927227404375No Hit
ACTTACTACTGTCAGCAGACTTACAGTACTCGATCGATCACCTTCGGCCC23490.18955946187385458No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23070.18617014837930293No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22580.18221594930232596No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22320.18011780285331777No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21610.1743882490887185No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21470.17325847792386795No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20850.1682552056223869No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20540.16575356947164638No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20500.1654307777102605No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20150.1626063497981341No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT19950.16099239099120471No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19900.16058890128947237No Hit
ACCTTGATGGGACCCCACTTTGCAAATTTGATGCAGCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTG19670.1587328486615036No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19600.15816796307907832No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA19440.15687679603353483No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19090.15405236812140843No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19070.1538909722407155No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGT18790.15163142991101436No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18620.1502595649251244No Hit
GGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTG18610.15017886698477795No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18530.1495332834620062No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18500.1492911896409668No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18470.1490490958199274No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18450.14888769993923445No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGTACTCGATCGATCAC18420.14864560611819505No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18410.1485649081778486No Hit
GTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGA18080.14590187614641512No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17840.14396512557809987No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA17790.14356163587636753No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17550.14162488530805228No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17530.14146348942735934No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17450.1408179059045876No Hit
AGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCCCTAACCTCTTGATCTATG17430.14065651002389465No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17390.1403337182625088No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17310.13968813473973704No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17190.1387197594555794No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17100.1379934779924612No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17050.13758998829072885No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16990.13710580064865005No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16870.13613742536449241No Hit
ATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA16810.1356532377224136No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16800.13557253978206715No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG16610.13403927891548423No Hit
GTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAA16610.13403927891548423No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT16430.13258671598924782No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT16160.13040787159989314No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCGTGTCTGCATCTGTAGGAGACAGAATCACCATCA16120.13008507983850728No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15890.1282290272105385No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15780.12734134986672735No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15770.12726065192638086No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAG15540.1254045992984121No Hit
GCAGAAACCAGGGAAAGTCCCTAACCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTT15470.12483971371598683No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15410.12435552607390803No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14860.11991713935485225No Hit
GGACCCCACTTTGCAAATTTGATGCAGCATAGATCAAGAGGTTAGGGACT14770.11919085789173403No Hit
TTACTACTGTCAGCAGACTTACAGTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGAA14670.11838387848826934No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14610.11789969084619054No Hit
CTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTT14590.1177382949654976No Hit
GGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGATT14390.11612433615856824No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG14360.11588224233752883No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGA14090.11370339794817418No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14020.11313851236574889No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCC13950.11257362678332361No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13860.11184734532020538No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCCCTAACCTCTTGATC13830.111605251499166No Hit
CCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGT13780.11120176179743366No Hit
GAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCC13750.11095966797639424No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13580.10958780299050426No Hit
CTCCAGTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAAT13440.10845803182565371No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13370.10789314624322843No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13360.10781244830288196No Hit
GCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTC13230.10676337507837788No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13110.10579499979422025No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12970.1046652286293697No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG12860.10377755128555853No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12780.10313196776278678No Hit
ATGCAGCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCT12770.10305126982244031No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC12660.10216359247862918No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12530.10111451925412507No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG12410.10014614396996746No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCACAGTGTTCATTTGCAGATACA12410.10014614396996746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA158.960912E-488.0224369
CGATTGT300.002821192465.9274270
AGTTCCG200.00559633255.45945467
TGTATAC200.006468462353.46595860
CGAAGGG400.00880904249.44556870
GTGGTAT2500.044.9537771
GGGCGTA504.2009397E-640.8670733
GTATCAA28850.040.1351971
TCTGGAG5700.034.6986470
TATCAAC32950.034.624352
CTTATAC3650.034.522411
GCGTTGT3850.034.46194562
ATCTCGT2600.034.36376637
ATGCCGT2550.033.99032244
CGTATGC2550.033.86926341
GAGTTTA2750.033.4366951
TCGTATG2600.033.1690540
CCCACGA3600.033.11524619
AATCTCG2700.033.06141736
GACAGGT3000.032.92071
Produced by FastQC (version 0.11.5)