FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239189
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63650.5136423903052723No Hit
GACTTACAGTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT50870.41051042254248543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47130.38032939285290623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46790.37758566288112627No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44350.35789536543658795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44050.3554744272261939No Hit
CTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC43960.3547481457630757No Hit
GTCTCCATCCTCCGTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG38960.31439917558984143No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37730.3044733289272258No Hit
ATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGT37400.30181029689579236No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37260.3006805257309418No Hit
GATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA34860.2813130200477893No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34380.2774395189111588No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34260.2764711436270012No Hit
GTCCCTAACCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT34170.275744862163883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33880.2734046218938354No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33850.273162528072796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32750.26428575463468446No Hit
GTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGA31900.25742642970523466No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31200.2517775738809818No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30970.24992152125301306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30260.2441919674884138No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC30060.2425780086814844No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30060.2425780086814844No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30020.24225521692009855No Hit
GCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT29780.2403184663517833No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28950.23362053730302643No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28310.22845586912085245No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGTACT27510.22200003389313494No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27340.220628168907245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27120.2188528142196227No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA26810.21635117806888216No Hit
CCTTCGGCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA26350.21263907281294459No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTG26040.21013743666220405No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25920.20916906137804647No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25680.2072323108097312No Hit
GGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTA25520.20594114376418768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25140.20287462203102188No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA24770.19988879823820255No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT24490.19762925590850144No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC24260.1957732032805327No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22230.17939152139019957No Hit
GATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAAT21860.17640569759738023No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21650.1747110408501044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21430.1729356861624821No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC21350.1722901026397103No Hit
ACTTACTACTGTCAGCAGACTTACAGTACTCGATCGATCACCTTCGGCCC21270.17164451911693857No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA21000.16946567472758392No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20740.16736752827857573No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20390.16454310036644937No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20230.16325193332090585No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19970.16115378687189766No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19810.1598626198263542No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19770.1595398280649683No Hit
GGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTG18780.1515507319706679No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18750.15130863814962853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18510.14937188758131326No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGT18140.14638606378849392No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18090.14598257408676157No Hit
ACCTTGATGGGACCCCACTTTGCAAATTTGATGCAGCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTG17850.14404582351844633No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17840.14396512557809987No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17800.14364233381671399No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17610.1421090729501311No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA17370.14017232238181584No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17330.13984953062042998No Hit
GTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGA17330.13984953062042998No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17190.1387197594555794No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17060.13767068623107534No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16840.135895331543453No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16520.13331299745236602No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16460.13282880981028722No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGACTTACAGTACTCGATCGATCAC16370.13210252834716898No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16300.13153764276474372No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16230.13097275718231843No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15980.12895530867365673No Hit
AGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCCCTAACCTCTTGATCTATG15960.1287939127929638No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15920.1284711210315779No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15900.12830972515088498No Hit
ATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA15780.12734134986672735No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15710.1267764642843021No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG15690.12661506840360914No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCGTGTCTGCATCTGTAGGAGACAGAATCACCATCA15630.1261308807615303No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15590.12580808900014445No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15490.12500110959667976No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT15330.12370994255113626No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15280.12330645284940392No Hit
GTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAA15120.12201528580386044No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT15110.12193458786351397No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG14840.1197557434741593No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14750.11902946201104109No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14580.11765759702515113No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14460.1166892217409935No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAG14200.11459107529198531No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14170.11434898147094591No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13900.11217013708159126No Hit
CTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTT13850.11176664737985892No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13740.11087897003604777No Hit
GGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGATT13690.11047548033431542No Hit
TTACTACTGTCAGCAGACTTACAGTACTCGATCGATCACCTTCGGCCCAGGGACACGACTGGAGATTAAACGAA13670.1103140844536225No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13670.1103140844536225No Hit
GCAGAAACCAGGGAAAGTCCCTAACCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGTT13600.1097491988711972No Hit
CCTCTTGATCTATGCTGCATCAAATTTGCAAAGTGGGGTCCCATCAAGGT13560.10942640710981134No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCCCTAACCTCTTGATC13430.10837733388530725No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13340.10765105242218902No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13120.10587569773456672No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13110.10579499979422025No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGA13050.10531081215214143No Hit
GGACCCCACTTTGCAAATTTGATGCAGCATAGATCAAGAGGTTAGGGACT13020.10506871833110203No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12960.10458453068902324No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCC12920.10426173892763735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG12760.10297057188209384No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12750.10288987394174737No Hit
GCCCAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTC12590.10159870689620389No Hit
ATGCAGCATAGATCAAGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCT12550.10127591513481801No Hit
CTCCAGTCGTGTCCCTGGGCCGAAGGTGATCGATCGAGTACTGTAAGTCTGCTGACAGTAGTAAGTTGCAAAAT12480.10071102955239272No Hit
GAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGTCC12420.10022684191031393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.052.1594661
CCTAACG200.00782959650.94522
GACAGGT2200.041.706021
GTATCAA25250.037.2800141
TATCAAC29550.031.8371582
CGGACTA4450.031.71619238
GTATTAC4600.030.99263
ATCAACG30350.030.7567543
AAGACCG450.005007921330.1690375
TCAACGC30900.030.095814
CAACGCA31600.029.5365355
GACTACT4800.029.49532140
GTGTATT5200.029.4080921
AACTAGC4750.029.38348226
CGCTGCG500.005846374729.22841566
AACGCAG32000.029.0612666
TAGCTCG4700.029.03053929
TCGTCCG4850.028.9475633
TTCGATC605.435478E-428.73815739
ATTCACT3100.028.4854892
Produced by FastQC (version 0.11.5)