FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985076
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35790.36332222082357096No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31600.3207874316296408No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA28520.2895208085467517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28190.28617081321644217No Hit
GTTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT24450.24820419947293404No Hit
CCCTTAGACTCTCCTGCGCAGCCTCTGGACTCACCTTTAGTATCGCCTGG21480.2180542415001482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21220.2154148512399043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20910.21226788592961354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20630.2094254656493509No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT19340.1963300293581409No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19170.19460427418798143No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACT18910.19196488392773756No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCAGTGGCCCTGGT18890.1917618539077188No Hit
GTGTATTGTCTCACCGTTAGCTTTGCTCTTAATAAGGGCAACCCACTCCAGCCCCTTACCTGGAGCCTGGCGGAC18560.18841185857740925No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18530.1881073135473811No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17900.18171186791679017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17800.18069671781669638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.1801891427666495No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC17690.1795800527065932No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17450.17714369246636807No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16970.17227097198591784No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16720.16973309673568335No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGG16700.16953006671566456No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16460.16709370647543945No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16080.163236136095083No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16000.16242401601500797No Hit
GGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCTGG15480.15714523549452022No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15390.1562316004044358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15180.15409978519423884No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15130.15359221014419192No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14960.15186645497403245No Hit
GTCTAAGGGACTCCCCAGGCTTTACCAAGGTTCCCCCAGACTCCACAAGT14940.1516634249540137No Hit
CCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGTAAGGGG14790.150140699803873No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14730.1495316097438167No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14650.14871948966374168No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14430.14648615944353532No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14410.14628312942351657No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14380.1459785843934884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14160.14374525417328204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13790.139989198802935No Hit
GACAATACACTACGCAGCACCCGTGAAAGACAGATTCACCATCTCAAGAGATGATTCAGAAAACAGTGTCTAT13430.13633465844259732No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA13330.13531950834250353No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA13240.1344058732524191No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12950.13146193796214709No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12880.1307513328920814No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12860.13054830287206268No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12790.129837697801997No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12580.12770588259180002No Hit
GTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12370.12557406738160304No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12370.12557406738160304No Hit
CCATAGATGAGGAGTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGC12350.12537103736158428No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11970.12151346698122785No Hit
GTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACTCCTCATCTATGGTG11940.12120892195119971No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11910.12090437692117158No Hit
CTCCCGGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA11910.12090437692117158No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTT11760.11938165177103086No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11730.11907710674100272No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11560.11735135157084327No Hit
GCCTCTGGACTCACCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGC11530.11704680654081512No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11490.11664074650077762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11170.11339226618047744No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAC11090.1125801461004024No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA11080.11247863109039302No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT11010.11176802602032737No Hit
TTATTGTACCACATGGGGATACAACTTTTTTACCTTTGACTATTGGGGCC10960.11126045097028046No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10920.11085439093024294No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10900.11065136091022419No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10870.11034681588019606No Hit
CTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10600.10760591060994279No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10590.10750439559993341No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10570.10730136557991464No Hit
CCTTGGGGCTGGTCGGGGATGCCGTGGAGACGGTGACCAGGGCTCCCTGG10540.1069968205498865No Hit
GTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAATTGCTGCTA10530.10689530553987711No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGG10500.10659076050984898No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10470.10628621547982084No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10370.10527106537972705No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10350.10506803535970828No Hit
GCCTTGGGATTCTCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA10340.1049665203496989No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTC10180.10334228018954883No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10150.10303773515952068No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10090.10242864509946441No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9870.10019531487925806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACT150.002455037368.2830436
TAAGGTA200.00795331650.74275628
CGTCCTA200.00805791550.57531418
CGTATGC1150.045.02776341
TCGTATG1150.044.93667240
GTGGTAT2000.042.1396751
TCGTCCC350.001426618738.974835
GGTATCA6600.038.819581
GTATCAA16650.038.064911
GCCGTCT1500.037.1860846
ACGAGAC1600.035.83691822
GTATGCC1500.034.575242
CTTATAC1600.033.7117421
GTATATC400.002907649833.7117421
TATCAAC18950.033.2669942
ATCTCCG1650.032.6918310
AACGCAG19700.032.0004846
ACGTACC951.1004886E-831.94068113
ATACGTA951.1004886E-831.94068111
TACGTAC951.1004886E-831.94068112
Produced by FastQC (version 0.11.5)