FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985076
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34840.3536782948726799No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33510.34017679854143235No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA26660.27063901668500706No Hit
GTTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT25750.2614011507741535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24400.24769662442288717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22310.22647998733092675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21130.21450121614981993No Hit
CCCTTAGACTCTCCTGCGCAGCCTCTGGACTCACCTTTAGTATCGCCTGG21080.213993641099773No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20380.2068875903991164No Hit
GTGTATTGTCTCACCGTTAGCTTTGCTCTTAATAAGGGCAACCCACTCCAGCCCCTTACCTGGAGCCTGGCGGAC19480.19775123949827222No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19250.1954163942680565No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT19010.19298003402783134No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18450.18729519346730608No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCAGTGGCCCTGGT18440.1871936784572967No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACT18280.18556943829714662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18080.18353913809695901No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18060.18333610807694026No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17300.1756209673162274No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC17180.17440278719611482No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGG16800.17054521681575838No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15680.15917553569470783No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15580.158160385594614No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15530.15765281054456712No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15310.15541948032436076No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG15270.15501342028432324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15060.15288160507412626No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14680.14902403469376982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14660.14882100467375106No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14650.14871948966374168No Hit
GGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCTGG14540.1476028245536385No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14420.14638464443352595No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14310.14526797932342275No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14180.14394828419330083No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14030.14242555904316012No Hit
CCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGTAAGGGG13940.1415119239530757No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13860.14069980387300066No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13840.1404967738529819No Hit
GTCTAAGGGACTCCCCAGGCTTTACCAAGGTTCCCCCAGACTCCACAAGT13760.13968465377290687No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13730.1393801087428787No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13580.13785738359273803No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA13240.1344058732524191No Hit
GTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13240.1344058732524191No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13070.13268011808225963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13020.13217254303221274No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12760.12953315277196886No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA12740.1293301227519501No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12660.12851800267187505No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12630.12821345764184694No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA12450.1263861874616781No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12420.12608164243164993No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12290.12476194730152801No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11840.1201937718511059No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11770.11948316678104023No Hit
CCATAGATGAGGAGTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGC11760.11938165177103086No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11760.11938165177103086No Hit
GACAATACACTACGCAGCACCCGTGAAAGACAGATTCACCATCTCAAGAGATGATTCAGAAAACAGTGTCTAT11720.11897559173099335No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTT11650.11826498666092768No Hit
GTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACTCCTCATCTATGGTG11560.11735135157084327No Hit
GCCTCTGGACTCACCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGC11350.11521953636064629No Hit
CTCCCGGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA11250.11420438626055249No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11230.11400135624053372No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11180.11349378119048684No Hit
GCCTTGGGATTCTCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA11090.1125801461004024No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA11040.11207257105035551No Hit
GTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAATTGCTGCTA10950.1111589359602711No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10870.11034681588019606No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10730.10892560574006473No Hit
CTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10650.10811348565998968No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTC10480.10638773048983023No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10480.10638773048983023No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10380.10537258038973642No Hit
TTATTGTACCACATGGGGATACAACTTTTTTACCTTTGACTATTGGGGCC10320.10476349032968015No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10280.10435743028964263No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10180.10334228018954883No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGG10060.10212410006943627No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10030.10181955503940812No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9990.1014134949993706No Hit
GTCCAGAGGCTGCGCAGGAGAGTCTAAGGGACTCCCCAGGCTTTACCAAGGTTCCCCCAGACTCCACAAGTTGCA9870.10019531487925806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)