FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749570
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34380.4586629667676134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34260.45706204890804064No Hit
GTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT34020.45386021318889497No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24510.3269874728177488No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22690.30270688528089434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20930.27922675667382635No Hit
GTAATAAACCCCAACATCCCCAGCCTCCACTTTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20120.26842056112170976No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG19960.2662860039756127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19410.2589484637859039No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19050.25414571020718546No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18780.2505436450231466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18530.2472083994823699No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18380.24520725215790387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17650.23546833517883586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.22786397534586494No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16150.21545686193417557No Hit
GGTCTAGTCAGAGCCTCCTGCACAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC16070.21438958336112707No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT15630.20851955120936003No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15380.20518430566858334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15250.20344997798737943No Hit
ATCCATTACTGTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15190.202649519057593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14530.1938444708299425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14220.1897087663593794No Hit
GATTTTACACTGAAAATCAGCAAAGTGGAGGCTGGGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACT13960.1862401109969716No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA13350.1781021118774764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13280.17716824312605894No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13030.17383299758528223No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12510.16689568686046666No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGA12470.1663620475739424No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12350.16476112971436957No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG12060.1608922448870686No Hit
CTACAAACTCCTGCGCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT12050.1607588350654375No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12010.16022519577891323No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11890.15862427791934045No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA11790.15729017970302975No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCC11630.15515562255693263No Hit
CTCCTGCACAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT11590.15462198327040835No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC11550.1540883439838841No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11500.15342129487572875No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC11460.15288765558920447No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCTGCGCTCACTTTCGGCGG11340.15128673772963167No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11260.1502194591565831No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10840.14461624664807823No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA10830.14448283682644716No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCC10760.1435489680750297No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10760.1435489680750297No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10280.13714529663673838No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10080.13447710020411702No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCGCCGCCGAAAGTG9930.132475952879651No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9800.13074162519844712No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9790.13060821537681605No Hit
CAGTAATAAACCCCAACATCCCCAGCCTCCACTTTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9670.1290072975172432No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9670.1290072975172432No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACT9650.12874047787398107No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9450.1260722814413597No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATCTCC9440.12593887161972867No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9190.12260362607895194No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9160.12220339661405873No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9120.12166975732753446No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAAAG9070.12100270821937911No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9040.12060247875448589No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9000.12006883946796164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8920.1190015608949131No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8910.11886815107328202No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8800.11740064303534027No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8720.11633336446229173No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8720.11633336446229173No Hit
TTATTACTGCATGCAAACTCTACAAACTCCTGCGCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAA8660.1155329055325053No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA8600.11473244660271889No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8460.11286470909988393No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8440.11259788945662179No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8390.11193084034846645No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8330.11113038141868004No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8260.11019651266726256No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8250.11006310284563149No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA8220.10966287338073828No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8180.10912923409421402No Hit
GTAATACAGAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATACA8180.10912923409421402No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGAATCCATTACTGTGC8160.10886241445095188No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8120.1083287751644276No Hit
CTCTACAAACTCCTGCGCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT8060.1075283162346412No Hit
CAGTAATACAGAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATA8050.10739490641301012No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8030.10712808676974798No Hit
GTATTACTGTACAAGAAACTATTACTATGATAGTCGTGGTTCTCGACCTGACAACTACTACTACGGTATGGACGT7890.10526034926691304No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7850.10472670998038876No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG7830.10445989033712662No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7730.10312579212081593No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7670.10232533319102953No Hit
AGTCAGAGCCTCCTGCACAGTAATGGATTCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG7610.1015248742612431No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG7560.10085782515308778No Hit
GTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA7550.10072441533145671No Hit
CTCCTGCGCTCACTTTCGGCGGCGGGACCAAGGTGGACATCAAACGAACT7530.10045759568819455No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7500.10005736622330137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG750.055.1899340
ACGCGTA750.054.29262527
GACGCGT750.054.2672126
GTCGTCC200.00753103351.44664437
GCGTAGT800.050.97095529
CGGCACA200.00795023850.74654813
TACCGCA200.00795023850.7465487
CGTATGC850.048.7998941
CGCGTAG850.047.9341228
AGACGCG850.047.85082625
GTAATCT800.046.9751734
ATGCCGT900.046.31607444
GGTATCA7250.045.7424281
ACGTCAA306.621978E-445.5516834
CGAGACG900.045.16528723
TAGTACC306.944568E-445.1140634
ATCTCGT903.6379788E-1241.91948337
TGCCGTC1000.041.76742645
GTAGTAA1100.040.2506331
GCCGTCT1050.039.83317646
Produced by FastQC (version 0.11.5)