FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225290
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34960.2853202099094908No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32900.2685078634445723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31210.2547152102767508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25380.20713463751438435No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23710.1935052110112708No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23160.18901647773180227No Hit
GTAATAGACGGCCGTGTCGTCGGGTCTCAGGTTGTCCATTTGGAGAAACA23120.18869002440238639No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA21770.17767222453459997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21590.17620318455222847No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21380.174489304572795No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20870.17032702462274235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20850.17016379795803444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19800.16159439806086723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19770.1613495580638053No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19570.15971729141672583No Hit
GAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCACTGAACCT18700.15261693150193018No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18440.15049498486072685No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18330.14959723820483314No Hit
CTCCTGTACAGTCTCTGGATCCACCTTCAGCGACTACTACATGAGCTGGCTCCGCCAGGCTCCAGGGAAGGGG18040.14723045156656792No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17870.14584302491655038No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17720.14461882493124076No Hit
GGCCAAAAGTGTACGGGGTTCTGAAACTCTGCTGACAGTAGTAAGATGCA17680.14429237160182487No Hit
GTCTATTACTGTGCGAGGGATTGGGGGGTTGGTGGCCGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT17260.140864611642958No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.14004847831941827No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17140.13988525165471033No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17090.13947718499294043No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTTACCATTACTTGCCGGTCAAGTCACAGAATTTCCAT16600.13547813170759576No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16560.13515167837817987No Hit
GAGTTACCATTACTTGCCGGTCAAGTCACAGAATTTCCATCTATGTGAATTGGTATCAACAGAGACCAGGGAAAG16300.13302973173697655No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG15770.12870422512221597No Hit
TCTTACTACTGTCAGCAGAGTTTCAGAACCCCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA15690.12805131846338416No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15690.12805131846338416No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15530.1267455051457206No Hit
CAGTAGTAAGATGCAAGATCTTCAGGTTGCAGAGAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCA15270.1246235585045173No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAATT15060.12290967852508386No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14660.11964514523092491No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14590.11907385190444711No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14550.11874739857503122No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14090.11499318528674844No Hit
GTCTGCATCTGTGGGAGACAGAGTTACCATTACTTGCCGGTCAAGTCACA13920.11360575863673089No Hit
GTAGTAAGATGCAAGATCTTCAGGTTGCAGAGAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCA13740.11213671865435938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTACGGGGTT13720.11197349198965142No Hit
CTGTTGATACCAATTCACATAGATGGAAATTCTGTGACTTGACCGGCAAG13380.10919863868961634No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13270.10830089203372262No Hit
GGTTAGGGGCTTTCCCTGGTCTCTGTTGATACCAATTCACATAGATGGAA13130.107158305380767No Hit
CCATCAACTCTCTGCAACCTGAAGATCTTGCATCTTACTACTGTCAGCAG13110.10699507871605905No Hit
TTACTACTGTCAGCAGAGTTTCAGAACCCCGTACACTTTTGGCCAGGGGA13040.10642378538958124No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13010.10617894539251933No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13000.10609733206016535No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12880.10511797207191768No Hit
ATCTGGGACAGACTTCAGTCTCACCATCAACTCTCTGCAACCTGAAGATC12500.1020166654424667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)