FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225290
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40370.3294730227129904No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33260.2714459434093154No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30670.25030809032963625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27580.22508957063225848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27570.22500795729990453No Hit
GTAATAGACGGCCGTGTCGTCGGGTCTCAGGTTGTCCATTTGGAGAAACA22960.1873842110847228No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22910.18697614442295293No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22410.18289547780525425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22320.18216095781406852No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21570.1760399578875205No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA21300.17383639791396321No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20870.17032702462274235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20700.16893959797272484No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18940.1545756514784255No Hit
GAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCACTGAACCT18440.15049498486072685No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18190.14845465155187754No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18080.1475569048959838No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18010.146985611569506No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17690.14437398493417883No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17660.14412914493711693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17630.143884304940055No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17290.14110945164001992No Hit
CTCCTGTACAGTCTCTGGATCCACCTTCAGCGACTACTACATGAGCTGGCTCCGCCAGGCTCCAGGGAAGGGG17120.13972202499000236No Hit
GTCTATTACTGTGCGAGGGATTGGGGGGTTGGTGGCCGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT17070.1393139583282325No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16700.13629426503113548No Hit
GGCCAAAAGTGTACGGGGTTCTGAAACTCTGCTGACAGTAGTAAGATGCA16690.13621265169878152No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16650.1358861983693656No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTTACCATTACTTGCCGGTCAAGTCACAGAATTTCCAT16610.13555974503994972No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16450.13425393172228614No Hit
GAGTTACCATTACTTGCCGGTCAAGTCACAGAATTTCCATCTATGTGAATTGGTATCAACAGAGACCAGGGAAAG15940.1300916517722335No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG15580.12715357180749048No Hit
GTCTGCATCTGTGGGAGACAGAGTTACCATTACTTGCCGGTCAAGTCACA15370.12543969182805703No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAATT15350.1252764651633491No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15190.12397065184568551No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14810.12086934521623452No Hit
TCTTACTACTGTCAGCAGAGTTTCAGAACCCCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA14100.11507479861910241No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13930.11368737196908486No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13920.11360575863673089No Hit
CAGTAGTAAGATGCAAGATCTTCAGGTTGCAGAGAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCA13850.11303446531025307No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13710.11189187865729745No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13490.11009638534551004No Hit
GTAGTAAGATGCAAGATCTTCAGGTTGCAGAGAGTTGATGGTGAGACTGAAGTCTGTCCCAGATCCACTGCCA13380.10919863868961634No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13120.10707669204841302No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13080.10675023871899714No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13080.10675023871899714No Hit
CCATCAACTCTCTGCAACCTGAAGATCTTGCATCTTACTACTGTCAGCAG12900.10528119873662561No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGTACGGGGTT12700.10364893208954615No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12530.1022615054395286No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12530.1022615054395286No Hit
CTGTTGATACCAATTCACATAGATGGAAATTCTGTGACTTGACCGGCAAG12510.10209827877482067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT200.006691313353.00994549
TACGTAT808.969364E-751.1345470
GTGGTAT2100.035.0320851
ATCAACG20100.031.9267753
CAACGCA20550.031.2327465
TCAACGC20800.030.8523164
GTACGTA903.29102E-530.2591369
AACGCAG21450.030.0830386
TCGGGTC7500.028.5702220
AGAGTAC22750.028.38370511
CGCAGAG22850.028.2548688
TCGTCGG7600.028.17816717
GCGTTGT8200.028.05442662
GTCGGGT7750.027.6429519
GGCGTTG8650.027.34260661
GGTACGT1056.4201176E-627.08596668
CGTTGTC8550.027.02352563
TGGTACG1006.570659E-627.00552667
TGTCGTC7950.026.9343215
GCCGATA500.00895358126.78458220
Produced by FastQC (version 0.11.5)