FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760639
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23740.31210600560844237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20800.273454293035198No Hit
GGGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC19840.26083332566434275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18770.24676620578224362No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA17520.23033265451810914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16520.2171858135068015No Hit
GTAGGAGACAGAGTCAACATCACTTGTCGGGCGAATCAGGGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAA15210.19996345178198857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14900.19588793106848323No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13960.18352990051785406No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13700.1801117218549141No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13680.17984878503468793No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12450.16367817059077958No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12280.1614432076188573No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12210.16052292874806576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12200.1603914603379527No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTGAGAGGGAAA12180.16012852351772655No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTG12080.1588138394165958No Hit
CTGTTAGCCTCTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11920.15671034485478658No Hit
GGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCAT11920.15671034485478658No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11830.15552712916376887No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11600.15250335573116813No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11560.15197748209071582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.1500054559390197No Hit
ATAATAGACAGCCGTGTCCTCACCTCTCAGGGAGTTCATTTGCAGATACA11290.1484278350176628No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAGAGGCTAACAGTTTC10790.14185441451200898No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10620.13961945154008668No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC10470.13764742538839056No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10200.1340977783153375No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC9610.126341142118666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9610.126341142118666No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTT9470.12450058437708295No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCAACATCACTTGTCGGGCGAATCAGGGTATTAGGAG9330.12266002663549988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9310.12239708981527372No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9250.12160827935459527No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9070.1192418479725599No Hit
CCTAATACCCTGATTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAG9020.11858450592199454No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8940.11753275864108993No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGCCGTAGTTAC8900.11700688500063763No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8820.11595513771973302No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT8480.11148521177588844No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8480.11148521177588844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8450.1110908065455492No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8430.11082786972532305No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8390.11030199608487075No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8350.10977612244441844No Hit
ATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA8080.10622647537136538No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC8030.10556913332080001No Hit
ACCTTGATGGGACCCCAGTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG8020.10543766491068693No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG7890.10372857557921696No Hit
GTCTATTATTGTGCGAAAGATCGCTCTGCGGTGACTTCCTACTACTTTGA7820.10280829670842541No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7790.10241389147808619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACGA400.00748228251.5262570
CGTATGC1650.050.141941
TCGTATG1650.050.08497240
ATCTCGT1500.048.0016237
GATCTCG1550.046.40072636
GTGGTAT1400.046.002961
CACGAGA1850.045.83376321
ACGAGAC2000.042.4018122
GGGTACT405.5422817E-542.37950518
CCCACGA2000.042.379519
CGAGCCC2000.042.3739315
GTATGCC2100.041.11021842
TCTCCGA2100.040.35612511
AGACCGA2150.039.47470525
CGAGACC2150.039.44873423
ATCTCCG2150.039.41761410
CTTATAC2150.039.415021
GTAATGC350.001469541138.7393343
TATACAC2300.038.3182563
TGCTTGA2150.037.9378655
Produced by FastQC (version 0.11.5)