FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760639
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21730.2856808551757141No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21150.2780556873891557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17500.23006971769788295No Hit
GGGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC16260.21376763484386155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15850.20837743002922543No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15780.2074571511584339No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14560.1914180051246386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13980.18379283733808022No Hit
GTAGGAGACAGAGTCAACATCACTTGTCGGGCGAATCAGGGTATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAA13190.17340683293914722No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13050.17156627519756415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12710.16709634925371955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12620.1659131335627019No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12120.15933971305704808No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTG12030.1581564973660304No Hit
CTGTTAGCCTCTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11700.15381803983229891No Hit
GGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCAT11300.14855930342777585No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11160.14671874568619278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTCCGCCGAAAGTGAGAGGGAAA11020.14487818794460972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10730.14106560405133053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10490.13791036220861672No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAGAGGCTAACAGTTTC10420.13699008333782517No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10360.1362012728771467No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9970.13107400488273677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9850.12949638396137983No Hit
ATAATAGACAGCCGTGTCCTCACCTCTCAGGGAGTTCATTTGCAGATACA9820.12910197873104062No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9750.12818169986024908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9660.1269984841692314No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9580.1259467368883268No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9420.12384324232651758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9250.12160827935459527No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9110.11976772161301222No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAACTGGGGTCCCATCAAGGTT8910.1171383534107507No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8840.11621807453995918No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA8500.1117481485961146No Hit
CCTAATACCCTGATTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAG8370.11003905926464459No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8340.10964465403430537No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATGCCGTAGTTAC8270.10872437516351384No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCAACATCACTTGTCGGGCGAATCAGGGTATTAGGAG8070.10609500696125232No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC7810.10267682829831234No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7750.10188801783763389No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7680.10096773896684234No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7670.10083627055672928No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7630.10031039691627698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCG200.006246227353.93842360
GTGGTAT2100.041.917441
GTATCAA16950.032.3583221
TACGAAA450.00452136830.8044944
TATCCGC450.00452136830.8044944
CTGCGAC450.004673957430.5962140
CTATCTA450.005067584630.09456820
ACGGGTA1152.0936568E-929.42290317
AATGGTA3201.8189894E-1228.97550470
TATCAAC18600.028.7502652
GCTATGA500.007125784628.06642750
ATCAACG19250.027.5836833
CGTAGGT500.00852927727.05126814
TCAACGC19650.027.0133024
CAACGCA20300.026.3148925
AACGCAG20300.026.3148926
CGGGTAC951.2189837E-524.93696218
ACGCAGA21600.024.8876517
GACAGGA1501.2951205E-924.828021
AGAGTAC22250.024.76515411
Produced by FastQC (version 0.11.5)