FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796758
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30980.38882571621496115No Hit
GCCTTGGGCTGACCCAGGACGGACAACTTGGTCCCTCCGCCGAATAGCAC22890.28728923964365594No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21480.2695925237023036No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18200.22842569512951236No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15970.20043727204496223No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15720.19729955645252387No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14730.18487420270646798No Hit
CCTATGAACATTCTGTAGGGACCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14120.17721817666091838No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13680.17169579721822686No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13430.16855808162578853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12860.16140409007502904No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12700.15939595209586851No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12530.15726230549301043No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11930.14973178807115836No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11860.14885322770527562No Hit
GTCATAGGACTGGCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGTCCAG11270.14144821890712111No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGACAACTTGGTCCCTC11220.14082067578863344No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11050.13868702918577536No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10600.1330391411193863No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10600.1330391411193863No Hit
GTCCCCAAACTCCTCATCTATGGTAACAACAATCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10580.13278812387199124No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10470.13140752901131839No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10320.12952489965585534No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10310.1293993910321578No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10310.1293993910321578No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10250.1286463392899726No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGAC9940.12475557195534906No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9440.11848014077047234No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9410.11810361489937975No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9360.11747607178089206No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9340.117225054533497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.1164720027913118No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8910.11182818371450302No Hit
GTCTGGGGCGCCAGGGCAGAGGGTCACCATCTACTGCAATGGGAGCAGCT8910.11182818371450302No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8850.11107513197231782No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8750.10982004573534249No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8540.10718436463769425No Hit
GTTTGGGGACTGCTCCTGGAAGTTGCTGGTACCAGTGTACATCATAATTT8530.10705885601399673No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8460.10618029564811399No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8360.10492520941113863No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGTCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC8310.10429766629265096No Hit
ATTCTGTAGGGACCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8240.10341910592676822No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8170.1025405455608855No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8090.10153647657130521No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8070.10128545932391014No Hit
GTCCTATGACAGCATCTCGAGTAGTCCGGTGCTATTCGGCGGAGGGACCA8060.1011599507002126No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8040.10090893345281754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATGG754.3052445E-544.22649470
GGTATCA13650.043.7997931
TCGTATG503.8675353E-641.35888340
TACGTAT859.006346E-539.0233870
GTATCAA35300.037.8923841
GTGGTAT3500.035.7079661
TCACGAT400.00227750335.43244652
TAACGGC652.4651383E-531.56586536
TTAACGG652.4877114E-531.52379835
ATCAACG42650.031.283083
TCAACGC42750.031.2099024
AACGCAG43500.030.749456
CAACGCA43650.030.6437845
TGGTATC4400.029.939422
TATCAAC45050.029.6914792
CGTATGC703.8150632E-529.61032941
CAGAGTA47050.028.35400410
CGCAGAG47300.028.3469458
AGAGTAC46950.028.3424611
ACGCAGA47250.028.3054667
Produced by FastQC (version 0.11.5)