FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347111
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT57890.42973444653038984No Hit
GTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA57110.42394427779151084No Hit
GTATTACTGTCAGCAGTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGA48820.36240517670778427No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47080.3494886464441312No Hit
CTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC46360.3441438753005506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40340.2994556499056128No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA38180.28342133647487106No Hit
CTCCTGTACAGGCTCTGGATTCATCTTCAGAACTTATGAAATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG37550.278744661724238No Hit
CTTTATTACTGTGCGACCCGATACTATGATAGTAGTCCTTTCGACCCCTGGGGTCAGGGAACCCTGGTCACCGT36540.27124713553671526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34460.2558066855663713No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT33210.24652756899765496No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32680.24259322357251928No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31640.2348729985873473No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28740.21334544814792544No Hit
GTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26890.1996123556262253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26510.19679150418933553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23970.177936339321704No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT23530.17467009028951586No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23410.1737792950989191No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23210.17229463644792448No Hit
GTGCTGGGCTTTCCTTGTTGCTATCTTAGAAGGTGTCCAGTGTGACGAGCCGCTTGTGGAGTCGGGGGGAGGC22870.16977071674123365No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC22790.1691768532808358No Hit
CATCTATGGTGCATCCAGAAGGGCCACTGGCATCCCAGACAGGATCAGTGGCAGTGGGTCTGGGACAGACTTCA22450.16665293357414498No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAGCTACTTAACCTGGTACC22430.1665044677090455No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG22300.165539439585899No Hit
GTGTATTACTGTCAGCAGTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAA21770.1616050941607633No Hit
GAGTTGGGGCTGTGCTGGGCTTTCCTTGTTGCTATCTTAGAAGGTGTCCA21690.16101123070036544No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT21590.16026890137486816No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20920.1552952948940362No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20560.1526229093222459No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20540.15247444345714645No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20250.15032168841320426No Hit
CCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGG19960.14816893336926207No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19800.1469812064484664No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGCTTTC19630.14571924659512098No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19160.14223029876528362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19150.14215606583273388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18500.1373309252170014No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG18420.13673706175660358No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18220.13525240310560896No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18040.13391621031971382No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17560.13035302955732675No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17470.12968493316437918No Hit
GTCCTAGGGAAACTACTATACTGCTGACAGTAATACACTGCAAAATCTTC17380.1290168367714316No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17370.12894260383888187No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCAGTGGCCCTTCT17280.1282745074459343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17020.1263444511996413No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC16860.1251567242788456No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT16780.12456286081844778No Hit
GCCTGGTACAGCCTGGAGGGTCCCTGAGACTCTCCTGTACAGGCTCTGGA16740.12426592908824886No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16620.12337513389765209No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16430.12196470817920721No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16130.1197377202027153No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16010.11884692501211852No Hit
CTATCATAGTATCGGGTCGCACAGTAATAAAGAGCCGTGTCCTCGGCTCT15960.11847576034936987No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC15940.1183272944842704No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15690.11647147117052715No Hit
CAGTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15610.11587760771012931No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15560.11550644304738064No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15530.11528374424973147No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG15360.11402178439638604No Hit
GTCAGAGTGTTAGCGGCAGCTACTTAACCTGGTACCAACAGAAGCCTGGC15200.11283405747559035No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCC15160.11253712574539143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14990.11127516589204603No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG14940.11090400122929736No Hit
GCCTGGAGGGTCCCTGAGACTCTCCTGTACAGGCTCTGGATTCATCTTCAGAACTTATGAAATGAACTGGGTCCG14900.11060706949909845No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATTTCATAAGTTC14750.10949357551085248No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC14700.10912241084810384No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGTTAAGTAGCTGCCGCTAACACTCTGA14660.1088254791179049No Hit
GGGCTGGAGTGGGTGTCATCCATTAGTAGTAGTGGTACTAGCATAAATTA14650.10875124618535519No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14560.1080831497924076No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG14180.10526229835551786No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14040.10422303729982162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13760.10214451518842915No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13720.10184758345823024No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG13570.10073408946998429No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC13520.10036292480723563No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGGGGTCGAAAGGAC13490.10014022600958643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1050.049.41142341
GGTATCA10850.046.4836851
TCGTATG1150.045.0810440
TCTGGAG18200.044.1227770
ATCTCGT1200.040.20671537
CGTAGGA350.001379309839.2442328
AGACGGA1350.038.13286225
CGAGACG1450.035.49773823
CACGAGA1450.035.49641821
TGCCGTC1500.034.69706745
AGCGAAC400.002660236834.32977327
CGAGCCC1600.034.31320615
CATCTCG1450.033.264636
GTATCAA25100.033.0791661
GTATGCC1500.032.31236342
CTTATAC1700.032.2911871
ACGAGAC1600.032.16863322
CGGCAAA800.003683669332.12137670
TACATCT1500.032.1174734
CTACATC1500.032.09954533
Produced by FastQC (version 0.11.5)