FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347111
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT54150.4019713297567906No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG50210.37272355433219684No Hit
GTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA50050.3715358274114011No Hit
GTATTACTGTCAGCAGTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGA47060.3493401805790317No Hit
CTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC43770.3249175457701704No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41190.30576544917233994No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA40030.2971544289965712No Hit
CTCCTGTACAGGCTCTGGATTCATCTTCAGAACTTATGAAATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG38580.28639065377686024No Hit
CTTTATTACTGTGCGACCCGATACTATGATAGTAGTCCTTTCGACCCCTGGGGTCAGGGAACCCTGGTCACCGT35390.26271034829349627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31690.23524416325009595No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30740.22819203465787155No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30490.2263362113441283No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28390.2107472955086849No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.20629331955570104No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27250.2022847411980156No Hit
GTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25730.19100133545045658No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24370.18090565662369323No Hit
GTGCTGGGCTTTCCTTGTTGCTATCTTAGAAGGTGTCCAGTGTGACGAGCCGCTTGTGGAGTCGGGGGGAGGC23970.177936339321704No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23670.1757093513452121No Hit
GTGTATTACTGTCAGCAGTATAGTAGTTTCCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAA22120.16420324680000387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22030.16353515040705627No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21700.16108546363291518No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG21680.16093699776781573No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT21640.1606400660376168No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21400.15885847565642325No Hit
GAGTTGGGGCTGTGCTGGGCTTTCCTTGTTGCTATCTTAGAAGGTGTCCA21240.15767074873562756No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20980.1557406924893346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20880.15499836316383728No Hit
CATCTATGGTGCATCCAGAAGGGCCACTGGCATCCCAGACAGGATCAGTGGCAGTGGGTCTGGGACAGACTTCA20660.1533652386477432No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAGCTACTTAACCTGGTACC20070.14898549562730912No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19870.1475008369763145No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC19550.14512538313472312No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19420.14416035501157662No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGCTTTC18720.1389640497330955No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT18030.13384197738716408No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17980.13347081272441544No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17920.13302541512911706No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17530.13013033075967756No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17510.12998186489457808No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGATGCCAGTGGCCCTTCT16910.12552788894159428No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16890.1253794230764948No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16820.1248597925486467No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16730.12419169615569911No Hit
CCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGG16430.12196470817920721No Hit
GTCCTAGGGAAACTACTATACTGCTGACAGTAATACACTGCAAAATCTTC16420.12189047524665747No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16320.12114814592116016No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16200.12025735073056341No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16120.11966348727016556No Hit
GCCTGGTACAGCCTGGAGGGTCCCTGAGACTCTCCTGTACAGGCTCTGGA15950.11840152741682014No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15790.11721380049602445No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15790.11721380049602445No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15610.11587760771012931No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15440.1146156478567839No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT15360.11402178439638604No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA15310.11365061973363738No Hit
GGGCTGGAGTGGGTGTCATCCATTAGTAGTAGTGGTACTAGCATAAATTA15240.11313098920578929No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC15160.11253712574539143No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15110.11216596108264278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15110.11216596108264278No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15080.1119432622849936No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14970.11112670002694656No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCC14840.11016167190380005No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14720.1092708767132033No Hit
GCCTGGAGGGTCCCTGAGACTCTCCTGTACAGGCTCTGGATTCATCTTCAGAACTTATGAAATGAACTGGGTCCG14650.10875124618535519No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14590.10830584859005679No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC14460.10734082046691029No Hit
GTCAGAGTGTTAGCGGCAGCTACTTAACCTGGTACCAACAGAAGCCTGGC14310.10622732647866434No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14290.10607886061356489No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATTTCATAAGTTC14210.10548499715316703No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGTTAAGTAGCTGCCGCTAACACTCTGA13810.10251567985117781No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC13740.1019960493233297No Hit
CTATCATAGTATCGGGTCGCACAGTAATAAAGAGCCGTGTCCTCGGCTCT13730.10192181639077998No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13730.10192181639077998No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG13710.10177335052568053No Hit
CAGTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA13630.10117948706528267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG200.00326565463.54169
GTGCCGT200.007542089651.4298631
GGTATCA9100.047.4737171
TCTGGAG20150.040.38416770
TACGTAT901.282425E-436.75433370
GTATCAA24400.036.6753921
TCCGAGA10850.034.7557170
AATGGTA3300.032.0765170
TATCAAC27950.031.883832
GTACGTA802.359461E-531.770569
CGCAATT450.00414325331.36100460
ATCAACG29100.030.6169973
TCAACGC29300.030.2899274
CAACGCA29800.029.8978045
AACGCAG30650.029.1804666
ATAGGTG14400.028.3172154
ATTCACT1700.028.2265662
CGGAACC500.007041291328.13672658
TAGGTGG14800.028.0159825
CGCAGAG32200.027.7758168
Produced by FastQC (version 0.11.5)