FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838432
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14370.17139135910843098No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTC13330.15898725239494677No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA13170.15707892828517994No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12910.15397790160680888No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT12790.1525466585244838No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12120.1445555513148353No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG12090.14419774054425402No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10620.12666501278577152No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10090.120343689172169No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10070.12010514865844815No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC9120.10877447425670776No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG9050.10793958245868478No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8760.10448074500973245No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8420.100425556276478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)