FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838432
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13180.15719819854204037No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12110.1444362810579749No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT12110.1444362810579749No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTC11790.14061963283844128No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA11090.1322707148582115No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10690.1274999045837945No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG10400.12404106713484218No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9360.11163696042135797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9270.11056352810961413No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8650.10316877218426777No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC8400.10018701576275715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT858.472428E-539.43091670
GTATCAA22950.037.7514531
GTGGTAT3200.036.3851971
CGGGTAT400.002707157534.20607817
TCAACGC26350.032.5795144
TATCAAC26600.032.4096262
ATCAACG26550.032.3398783
CAACGCA26900.031.9114865
AACGCAG27300.031.4439166
ACGCAGA28650.029.9622657
CGCAGAG29100.029.851518
AGAGTAC29800.028.9173211
GCAGAGT30100.028.632529
GGTATCA15250.027.845031
CAGAGTA31200.027.6197510
GCACGAA1258.143654E-426.81302570
TTCGTAT1000.00890100826.8130270
GCCCGAT801.0151733E-425.64690812
ACGGGGT2550.025.48536116
GAGTACG25800.024.78538512
Produced by FastQC (version 0.11.5)