FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270276
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA92680.7296052196530517No Hit
GTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT67300.5298061208745186No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62840.4946956409473217No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54810.4314810324685344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39840.31363262786984875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39720.31268795127987936No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36680.2887561443339873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35540.27978171672927776No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35270.2776561944018465No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34360.2704923969279117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33870.2666349675188699No Hit
GTTTCAATACTTCGCCTACTTTCGGCCCTGGGACCAAAGTGGATATCAGA30050.23656276273817659No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGGCCCAGTC29680.2336500099191042No Hit
GTATTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT29660.23349256382077596No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29310.2307372571000318No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29300.23065853405086767No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29150.2294776883134059No Hit
GTGTTAGTACGTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATACTGCATCCA27850.21924369192207047No Hit
GTAATAAACAGCCGTGTCATCTTCTCTCAGGCTGTTCATTTGCAGATACA27750.21845646143042927No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTA27710.21814156923377284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27410.21577987775884927No Hit
ATACTGCATCCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA26710.21026926431736095No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26680.21003309516986862No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26600.20940331077655563No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCACTT26030.20491609697420088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25560.2012161136634873No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT25050.19720123815611723No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24660.19413103923871664No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24380.1919267938621213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24040.18925021019054125No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23630.18602256517481242No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23290.18334598150323236No Hit
ACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAA23180.18248002796242707No Hit
GTGCCAGATGTGTCATCCAGATGGCCCAGTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCA22440.17665452232428228No Hit
GTTTATTACTGTGCGAGCGAACCGGGTTTAGCAGGAGCTGGGCGGCTTGA20890.1644524497038439No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20740.1632716039663821No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20630.1624056504255768No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20480.161224804688115No Hit
GTAGGAGACACAGTCAGCATCACTTGCCGGGCAAGTCAGAGTGTTAGTACGTATTTAAATTGGTATCAGCAGAAA20400.16059502029480208No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20350.16020140504898148No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA20210.15909928236068382No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19840.15618652954161144No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC19560.1539822841650161No Hit
GCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTAGGCGA19440.1530376075750467No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19290.1518567618375849No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC19220.15130570049343608No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTTCGGCCCTGGGA19180.1509908082967796No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19140.15067591610012313No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA19100.15036102390346665No Hit
CACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTT18200.14327594947869596No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18000.14170148849541359No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17820.14028447361045948No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17570.13831639738135648No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17390.13689938249640235No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17370.1367419363980741No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGGCCCAGTCTCCATCTTCC17340.13650576725058175No Hit
ATGCAGTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATTTAAATACGTACTAACACT17140.1349313062672994No Hit
GTCTGCATCTGTAGGAGACACAGTCAGCATCACTTGCCGGGCAAGTCAGA17040.1341440757756582No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16990.1337504605298376No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16340.1286334623341699No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16230.1277675087933646No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCACTTGCCG15960.1256419864659334No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15920.12532709426927693No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15660.12328029499100984No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15650.12320157194184571No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15600.12280795669602511No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15550.12241434145020451No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15460.12170583400772746No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15340.12076115741775804No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15330.12068243436859391No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15240.11997392692611684No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15010.11816329679534211No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14770.11627394361540327No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG14740.11603777446791091No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14710.11580160532041857No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14630.11517182092710561No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTG14580.11477820568128502No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14560.11462075958295678No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14550.11454203653379266No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC14480.11399097518964382No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14350.11296757555051028No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14340.11288885250134617No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14120.11115694541973554No Hit
ACCTTGATGGGACCCCAGTTTGCAAAGTGGATGCAGTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTG14060.11068460712475084No Hit
GTCAGAGTGTTAGTACGTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATACTG14050.11060588407558673No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13930.10966120748561729No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC13920.10958248443645319No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13890.10934631528896083No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG13720.1080080234531708No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13530.10651228551905255No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13350.10509527063409842No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13310.10478037843744195No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13240.10422931709329311No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13220.10407187099496487No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA13200.10391442489663663No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13120.10328464050332369No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA13020.10249741001168251No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12910.10163145647087718No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12900.10155273342171307No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12900.10155273342171307No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT12900.10155273342171307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG9600.042.09817570
CTAGCGC857.8216544E-1139.98616428
GTGGTAT2000.037.2840271
TGCGGCA609.345231E-636.373363
GTATCAA20750.035.2830241
TCGTATG1003.8926373E-1034.43823640
AATGGTA6150.033.39575270
CGTATGC1056.475602E-1032.83904341
TAGCGCT1057.4942363E-1032.39148729
TGACTTG29600.032.00729470
TATCAAC22700.031.6548422
ACTAGCG1101.2514647E-930.8801127
GACTAGC1101.2569217E-930.8642826
ATCAACG23200.030.8265293
TCAACGC23450.030.4990884
AACGCAG23700.030.1773686
ACACCGT450.00504002430.1296966
CAACGCA23750.030.1138345
TGTTAGA13000.030.0686170
ATTCACT2150.029.9533422
Produced by FastQC (version 0.11.5)