FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270276
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA85330.6717437785174246No Hit
GTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATT63750.5018594384212565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63010.49603393278311164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45030.35448989038602635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42220.33236871357090897No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39350.3097751984608069No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37900.29836035633200975No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37460.2948965421687885No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32700.257424370766668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32360.254747787095088No Hit
GTTTCAATACTTCGCCTACTTTCGGCCCTGGGACCAAAGTGGATATCAGA29830.23483085565656597No Hit
GTATTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAAT28940.22782450428095943No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28560.2248330284127229No Hit
GTGTTAGTACGTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATACTGCATCCA28360.22325856742944056No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28210.22207772169197873No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27900.2196373071678911No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27650.21766923093878812No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTA27020.21270967884144862No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGGCCCAGTC26870.21152883310398685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26700.21019054126819683No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26610.20948203382571978No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCACTT26060.20515226612169324No Hit
GTAATAAACAGCCGTGTCATCTTCTCTCAGGCTGTTCATTTGCAGATACA25970.2044437586792162No Hit
ATACTGCATCCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA25210.19846080694274315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25080.1974374073036096No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24190.19043105592800305No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23730.1868097956664536No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT23070.18161407442162178No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22560.1775991989142517No Hit
GTGCCAGATGTGTCATCCAGATGGCCCAGTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCA22410.17641835317678994No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22150.17437155389852285No Hit
ACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAA22000.17319070816106105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21960.17287581596440457No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21720.17098646278446572No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC21660.17051412448948103No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21610.17012050924366043No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21090.16602691068712624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21000.1653184032446492No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20310.159886512852325No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20240.15933545150817616No Hit
GTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTTCGGCCCTGGGA19950.15705248308241673No Hit
GTAGGAGACACAGTCAGCATCACTTGCCGGGCAAGTCAGAGTGTTAGTACGTATTTAAATTGGTATCAGCAGAAA19510.1535886689191955No Hit
GTAGTAAGTTGCAAAATCCTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18870.1485503937726919No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA18740.14752699413355835No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18720.14736954803523014No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC18680.14705465583857366No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18330.14429934911782952No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18270.1438270108228448No Hit
GTTTATTACTGTGCGAGCGAACCGGGTTTAGCAGGAGCTGGGCGGCTTGA18270.1438270108228448No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18260.14374828777368068No Hit
GCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTAGGCGA17360.13666321334891No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17140.1349313062672994No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGGCCCAGTCTCCATCTTCC17130.13485258321813526No Hit
GTCTGCATCTGTAGGAGACACAGTCAGCATCACTTGCCGGGCAAGTCAGA17040.1341440757756582No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16990.1337504605298376No Hit
CACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTTCAATACTTCGCCTACTTT16950.13343556833318115No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16940.133356845284017No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16870.1328057839398682No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16750.13186110734989875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16650.13107387685825758No Hit
ATGCAGTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATTTAAATACGTACTAACACT16610.1307589846616011No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16180.127373893547544No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16000.12595687866258987No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15840.12469730987596395No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15810.1244611407284716No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15390.12115477266357862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15140.11918669643447566No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC15040.11839946594283447No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15020.11824201984450622No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACACAGTCAGCATCACTTGCCG15000.118084573746178No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14740.11603777446791091No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14710.11580160532041857No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14550.11454203653379266No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14500.11414842128797206No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14220.11194417591137674No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14120.11115694541973554No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14080.11084205322307908No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14080.11084205322307908No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13920.10958248443645319No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13880.10926759223979671No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13750.10824419260066316No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG13710.1079293004040067No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13330.10493782453577018No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13310.10478037843744195No Hit
GTCAGAGTGTTAGTACGTATTTAAATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATACTG13160.10359953269998017No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTG13140.10344208660165193No Hit
ACCTTGATGGGACCCCAGTTTGCAAAGTGGATGCAGTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTG13140.10344208660165193No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAGGATTTTGCAACTTA13000.10233996391335427No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12990.10226124086419014No Hit
GTGGATGCAGTATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATTTAAATACGTACTAACA12980.10218251781502602No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12950.10194634866753367No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG12920.10171017952004131No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12900.10155273342171307No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12880.10139528732338483No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12860.10123784122505661No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12830.10100167207756423No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12790.10068677988090777No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12710.10005699548759482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)